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We narrowed to 11,503 results for: ENA

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Showing: 5361 - 5380 of 11503 results

  4. pET-NlaCascade-NLS-6xHis

    Plasmid
    #180213
    Purpose
    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli
    Depositor
    Yan Zhang
    Article
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Insert
    NlaCas5-Cas8-Cas7
    Tags
    6XHis and NLS
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180213-plasmid-map-sequence-id-356902

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  5. pRSF-MjCouRS

    Plasmid
    #229066
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024
    Insert
    Methanococcus jannaschii tRNA synthetase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    229066-plasmid-map-sequence-id-459819

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  8. pCSN068

    Plasmid
    #101749
    Purpose
    Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available Since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101749-plasmid-map-sequence-id-202306

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  12. pEM-Cas9HF1

    Plasmid
    #89961
    Purpose
    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
    Depositor
    Michael Lynch
    Article
    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021
    Insert
    Cas9HF1
    Expression
    Bacterial
    Mutation
    N497A/R661A/Q695A/Q926A
    Promoter
    pTet
    Available Since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    89961-plasmid-map-sequence-id-181339

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  13. LSB-hsa-let-7a-5p

    Plasmid
    #103146
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7a-5p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103146-plasmid-map-sequence-id-213119

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Showing: 5361 - 5380 of 11503 results