We narrowed to 14,670 results for: nts

1. pGEX nsp1 CoV2

   Plasmid

   #175512

   Purpose

   For protein expression of WT nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 CoV2
   

   Tags

   GST tag

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-LiMETER-v2

   Plasmid

   #113934

   Purpose

   Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

   Depositor

   Yubin Zhou

   Article

   He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

   Insert

   LOV2 and Stim1 polybasic domain (PB)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAF0688 Kp03_attP-mCherry donor

   Plasmid

   #193467

   Purpose

   Promoterless mcherry donor for Kp03

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Kp03_attP
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAF0690 Ef1a-Kp03_attB-BFP acceptor

   Plasmid

   #193473

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Kp03_attB
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYO1125

   Plasmid

   #235742

   Purpose

   Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   p40 PX domain (NCF4 Human)
   

   Tags

   GST-TEV

   Expression

   Bacterial

   Mutation

   aa 2-149 with Cys at 2

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP2292

   Plasmid

   #70707

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available Since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. LiPOP1

   Plasmid

   #168264

   Purpose

   A single-component optogenetic tool for photoswitchable necroptosis

   Depositor

   Yubin Zhou

   Article

   He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

   Insert

   MLKL(1-125) (MLKL Human)
   

   Tags

   mCherry, CRY2-PHR

   Expression

   Mammalian

   Mutation

   H15A, K16A, R17A

   Promoter

   CMV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-mCherry-p2A-full length LIC1

   Plasmid

   #74601

   Purpose

   expresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherry

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   full length human dynein light intermediate chain 1 (DYNC1LI1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAF0443 Nm60_attP-mCherry donor

   Plasmid

   #193471

   Purpose

   Promoterless mcherry donor for Nm60

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Nm60_attP
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAF0466 Ef1a-Nm60_attB acceptor

    Plasmid

    #193476

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Nm60_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. BPK1179

    Plasmid

    #179296

    Purpose

    Expresses dCas9 fused to four DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-DmrA(x4)
    

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-OptoPB

    Plasmid

    #113935

    Purpose

    Express a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactions

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    OptoPB
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. cpLOV2-V7

    Plasmid

    #168993

    Purpose

    circularly permuted LOV2 variant-V7

    Depositor

    Yubin Zhou

    Article

    He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

    Insert

    cpLOV2-V7
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJP-HBB-nLuc

    Plasmid

    #175431

    Purpose

    Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' HBB leader
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO CBX1-APEX2

    Plasmid

    #129642

    Purpose

    Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells

    Depositor

    Nicholas Ingolia

    Article

    Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

    Insert

    CBX1 (CBX1 Human)
    

    Tags

    1xFLAG and APEX2

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPK2301

    Plasmid

    #65778

    Purpose

    Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    St1Cas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP977

    Plasmid

    #65774

    Purpose

    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E mutation in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAF0185 Cp36_attP-mCherry donor

    Plasmid

    #193468

    Purpose

    Promoterless mcherry donor for Cp36

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. BPK2797

    Plasmid

    #72251

    Purpose

    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335Q, and T1337R

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. cpLOV2-V5

    Plasmid

    #168991

    Purpose

    circularly permuted LOV2 variant-V5

    Depositor

    Yubin Zhou

    Article

    He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

    Insert

    cpLOV2-V5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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