We narrowed to 14,164 results for: nts

1. pYO979

   Plasmid

   #235756

   Purpose

   Protein expression of ScVPS38, untagged + ScVPS30 FRK to DDD at 430-432, untagged

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Inserts

   VPS38 (VPS38 Budding Yeast)
   VPS30 (VPS30 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   FRK430-432DDD

   Promoter

   Available since

   July 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRAR120

   Plasmid

   #185041

   Purpose

   J23108 promoter, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Only first 90 nt of rpmE coding present

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p667-UBC-MAFG-V5-miniTurbo

   Plasmid

   #207818

   Purpose

   C-terminal tagged MAFG for miniTurbo proximity experiments

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   MAFG-V5-miniTurbo
   

   Use

   Lentiviral

   Tags

   V5-miniTurbo

   Expression

   Mutation

   Promoter

   UBC

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p663-UBC-miniTurbo-V5-MAFG

   Plasmid

   #207817

   Purpose

   N-terminal tagged MAFG for miniTurbo proximity experiments

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   miniTurbo-V5-MAFG
   

   Use

   Lentiviral

   Tags

   miniTurbo-V5

   Expression

   Mutation

   Promoter

   UBC

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-Cas9-NS-A

   Plasmid

   #207823

   Purpose

   Dual expression of Cas9 and non-specific sgRNA

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   non-specific sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   EF-1a; U6

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-Cas9-NS-B

   Plasmid

   #207824

   Purpose

   Dual expression of Cas9 and non-specific sgRNA

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   non-specific sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   EF-1a; U6

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1-ZNF746-C571A

   Plasmid

   #207826

   Purpose

   Dox-inducible expression of ZNF746-C571A

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   ZNF746-C571A (ZNF746 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   C571A

   Promoter

   TRE

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUMO human-cPLA2-C2-domain

   Plasmid

   #170752

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

   Insert

   PLA2G4A-C2 domain (PLA2G4A Human)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. XE57 Delta N-cadherin

   Plasmid

   #17025

   Depositor

   Randall Moon

   Insert

   dominant N-cadherin
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   nt 90-2004 deleted

   Promoter

   Available since

   April 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGNT-Sema3A

    Plasmid

    #16428

    Depositor

    Jonathan Raper

    Insert

    Sema3A (SEMA3A Chicken)
    

    Use

    Tags

    His and Myc

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

11. NK691

    Plasmid

    #120850

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Insert

    Jumblelw1-mWasabi
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Jumblelw1 allele: T to C at nt 1109 causes L305P …

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSP1673

    Plasmid

    #65769

    Purpose

    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti SYN-dCas9-KRAB-MeCP2

    Plasmid

    #155365

    Purpose

    Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

    Depositor

    Jeremy Day

    Article

    Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    3x FLAG and 7x His

    Expression

    Mammalian

    Mutation

    Promoter

    SYN

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAF0033 Ef1a-Pa01-2a-EGFP

    Plasmid

    #193460

    Purpose

    Expression of Pa01 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAF0034 Ef1a-Kp03-2a-EGFP

    Plasmid

    #193461

    Purpose

    Expression of Kp03 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAF0036 Ef1a-Si74-2a-EGFP

    Plasmid

    #193463

    Purpose

    Expression of Si74 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAF0432 Ef1a-Dn29-2a-EGFP

    Plasmid

    #193459

    Purpose

    Expression of Dn29 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Dn29
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

    Plasmid

    #193465

    Purpose

    Dn29 Donor with promoter for mcherry and puro expression

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Dn29_attP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-mOXT-hM3Dq-mCherry-WPRE

    Plasmid

    #70717

    Purpose

    Expresses DREADDs Gq receptor under oxytocin promoter control

    Depositor

    Daniel Geschwind

    Article

    Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    mouse oxytocin

    Available since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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