We narrowed to 11,578 results for: VARS

1. pPB-cT3G-cERP2-PPARG

   Plasmid

   #192912

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of PPARG

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   PPARG (PPARG Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTwist-SARS-CoV-2 Δ18 B.1.1.7

   Plasmid

   #169459

   Purpose

   Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-SopB

   Plasmid

   #183657

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

   Plasmid

   #139995

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   HF1=N497A/R661A/Q695A/Q926A

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pαH-S-GSAS

   Plasmid

   #164565

   Purpose

   SARS-CoV-2 Spike protein (S-GSAS variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-GSAS variant)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

   Plasmid

   #233039

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   RfxCas13d-compatible gRNA expression cassette and hfCas13d
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-cT3G-cERP2-NR1H2

   Plasmid

   #192919

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   NR1H2 (NR1H2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MS2_adRNA-MCP_ADAR2 DD_NES

   Plasmid

   #124706

   Purpose

   Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NES

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   MCP_ADAR2 DD_NES (RAB7A Human)
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MS2_adRNA-MCP_ADAR1 DD_NES

   Plasmid

   #124630

   Purpose

   Expresses MS2_adRNA targeting the RAB7A transcript along with MCP_ADAR1 DD_NES

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   MCP_ADAR1 DD_NES (RAB7A Human)
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-RR-ABE8e-V106W

    Plasmid

    #193656

    Purpose

    Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA*8e-V106W, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-G532R/K595R, TadA*8e-V106W

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_BCAR1-1/1

    Plasmid

    #91418

    Purpose

    Protein expression and purification of human SH3 domain construct BCAR1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BCAR1-1/1 (BCAR1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX VAMP7 (1-188)

    Plasmid

    #42315

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Δ181-220

    Promoter

    Ptac

    Available Since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pT2-TetR-neoR

    Plasmid

    #153310

    Purpose

    Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Insert

    TetR
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_FYN-1/1

    Plasmid

    #91413

    Purpose

    Protein expression and purification of human SH3 domain construct FYN-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FYN-1/1 (FYN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEMS2214

    Plasmid

    #158417

    Purpose

    AAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple265 (PCP2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple265

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDF-Az

    Plasmid

    #197101

    Purpose

    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

    Promoter

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

    Plasmid

    #119723

    Purpose

    AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Thomas Oertner

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Inserts

    CaMPARI2
    CaMPARI2
    

    Use

    AAV

    Tags

    NES-his

    Expression

    Mammalian

    Mutation

    F391W, L398V

    Promoter

    hSyn1

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pαH-S-RRAR/D614G

    Plasmid

    #164570

    Purpose

    SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-RRAR-D614G variant)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEMS2284

    Plasmid

    #158419

    Purpose

    AAV plasmid with Ple349 (PDE6H MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple349 (PDE6H Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple349

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLL5.0-Anillin shRNA

    Plasmid

    #187270

    Purpose

    Lentiviral expression of shRNA targeting Anillin

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    shRNA for Anillin (ANLN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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