167,318 results

1. pLenti SpBsmBI sgRNA Puro

   Plasmid

   #62207

   Purpose

   An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

   Depositor

   Rene Maehr

   Article

   Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-PE6e

   Plasmid

   #207855

   Purpose

   Mammalian expression of PE6e prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6e
   

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Cas9(H840A, K918A, K775R)

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mRFP-Rab5

   Plasmid

   #14437

   Purpose

   Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

   Depositor

   Ari Helenius

   Article

   Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

   Insert

   Rab 5 (RAB5A Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. RalGDS-YPet

   Plasmid

   #134929

   Purpose

   Fluorescently tagged affinity reagent

   Depositor

   Klaus Hahn

   Article

   O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

   Insert

   RaIGDS RBD, YPet
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28b-RfxCas13d-His

   Plasmid

   #141322

   Purpose

   Plasmid for bacterial expression and purification of RfxCas13d protein

   Depositor

   Ariel Bazzini, Miguel Angel Moreno-Mateos

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. LOTUS-V/pcDNA3

   Plasmid

   #87127

   Purpose

   Bioluminescent and ratiometric voltage indicator

   Depositor

   Takeharu Nagai

   Article

   Inagaki et al Sci Rep. 2017 Feb 13;7:42398. doi: 10.10

   Insert

   LOTUS-V
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTT3-ecdICAM1-ST3-His

   Plasmid

   #210666

   Purpose

   Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)

   Depositor

   Omer Dushek

   Article

   Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

   Insert

   Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Human, Synthetic)
   

   Tags

   Histag and Spytag003

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGJK_His-SUMO-LbuCas13a

   Plasmid

   #172488

   Purpose

   Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

   Insert

   bdSUMO-LbuCas13a (codon-optimized)
   

   Tags

   His6 and bdSUMO

   Expression

   Bacterial

   Promoter

   T7 promoter and lac operator

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-Rex

   Plasmid

   #122134

   Purpose

   B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 origin

   Depositor

   Srivatsan Raman

   Article

   Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

   Insert

   B. subtilis Rex
   

   Expression

   Bacterial

   Promoter

   apFAB338

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK485 (3mAID-Hygro)

    Plasmid

    #214397

    Purpose

    3mAID for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    3mAID-Hygro
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-HIF2alpha-pcDNA3

    Plasmid

    #18950

    Depositor

    William Kaelin

    Article

    Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG-CyclinF-ΔF

    Plasmid

    #32973

    Depositor

    Michele Pagano

    Article

    D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

    Insert

    CYCLIN F (CCNF Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F-box mutations: L35A and P36A

    Promoter

    CMV

    Available Since

    Feb. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (AAV1)

    Viral Prep

    #100851-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (#100851). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Promoter

    Syn

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5FRT-EF-Pdgfralpha-EGFPN

    Plasmid

    #66787

    Purpose

    Expression of murine PDGFRalpha tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRalpha (Pdgfra Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDP085

    Plasmid

    #198425

    Purpose

    pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120

    Depositor

    Markus Schmid

    Article

    You et al Nat Commun. 2017 May 17;8:15120. doi: 10

    Insert

    E. coli BirA
    

    Expression

    Plant

    Promoter

    pUBQ10

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMF440

    Plasmid

    #62550

    Purpose

    Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.

    Depositor

    Michael Franklin

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG241 FKBP-CFAST10

    Plasmid

    #130814

    Purpose

    Expresses FKBP-CFAST10 in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

    Insert

    FKBP-CFAST10
    

    Tags

    Myc-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGPTVII-Bar-U-MatryoshCaMP6s

    Plasmid

    #100024

    Purpose

    Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    MatryoshCaMP6s
    

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOTTC841 - pAAV minP secNLuc

    Plasmid

    #82498

    Purpose

    Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

    Depositor

    Brandon Harvey

    Article

    Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

    Insert

    secNLuc
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    c-Fos minimal promoter

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. EF1a-EGFP-SKL

    Plasmid

    #170166

    Purpose

    Mammalian Expression of Ef1a-EGFP-SKL

    Depositor

    Christopher Moxham

    Insert

    EGFP-SKL
    

    Expression

    Mammalian

    Mutation

    SKL

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. CFP-GAI(1-92)

    Plasmid

    #37307

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available Since

    June 19, 2012

    Availability

    Academic Institutions and Nonprofits only

22. AB-GFP

    Plasmid

    #72203

    Purpose

    screen AB42 variants for aggregation

    Depositor

    Michael Hecht

    Article

    Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

    Insert

    AB42 (APP Human)
    

    Tags

    GFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLEW100cre-EP1

    Plasmid

    #33345

    Purpose

    For expression of tetracycline-inducible CRE in Trypanosoma brucei

    Depositor

    George Cross

    Article

    Scahill et al Mol Biochem Parasitol. 2008 Jan;157(1):7

    Insert

    cre recombinase
    

    Use

    Cre/Lox; Trypanosoma brucei expression vector

    Promoter

    TET operator

    Available Since

    Dec. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMyC

    Plasmid

    #42192

    Depositor

    Annabel Parret, Matthias Wilmanns

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Promoter

    ColE1

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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25. LEGO-AP1x6-GM55

    Plasmid

    #217415

    Purpose

    Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x6

    Depositor

    Peter Cockerill

    Insert

    Luciferase, GFP
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. LentiCRISPR v2-sgLKB1 clone2

    Plasmid

    #162126

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pJFRC7-syt1-GCaMP6f

    Plasmid

    #190896

    Purpose

    GCaMP6f fused to Drosophila synaptotagmin1 via a 3x GS linker. In the pJFRC7-20XUAS vector.

    Depositor

    Tom Clandinin

    Article

    Turner et al Elife. 2022 Oct 27;11. pii: 82587. doi:

    Insert

    syt1-GCaMP6f
    

    Expression

    Insect

    Available Since

    Nov. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCW57-MCS1-P2A-MCS2 (Blast)

    Plasmid

    #80921

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. GFP-P4M-SidM

    Plasmid

    #51469

    Purpose

    Biosensor for PtdIns4P

    Depositor

    Tamas Balla

    Article

    Hammond et al J Cell Biol. 2014 Apr 7.

    Insert

    P4M-SidM(546-647)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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30. mCherry-hLC3B-pcDNA3.1

    Plasmid

    #40827

    Depositor

    David Rubinsztein

    Article

    Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

    Insert

    LC3B (MAP1LC3B Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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