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We narrowed to 14,239 results for: CAN

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Showing: 5541 - 5560 of 14239 results

  1. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid
    #99690
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Synthetic, Mouse)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available Since
    Sept. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    99690-plasmid-map-sequence-id-194510

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  4. p37-2iDMD-LR

    Plasmid
    #88892
    Purpose
    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    mCherry
    Expression
    Mammalian
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    88892-plasmid-map-sequence-id-451853

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  5. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  11. pBZCas13b

    Plasmid
    #89898
    Purpose
    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89898-plasmid-map-sequence-id-178222

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  12. Nluc CD63

    Plasmid
    #242528
    Purpose
    This plasmid can be used to quantify CD63 by luminescence signal upon providing substrate, in particular in the secretome, especially in the extracellular vesicles of cells expressing this construct.
    Depositor
    Clotilde Thery
    Article
    Grisard et al J Extracell Vesicles. 2022 Jul;11(7):e12
    Insert
    CD63 (CD63 Human)
    Use
    Luciferase
    Tags
    Nanoluciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    242528-plasmid-map-sequence-id-500759

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  16. pCAGGS-FuG-B2

    Plasmid
    #67513
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.
    Insert
    Fusion Glycoprotein type B2
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Promoter
    CAGGS
    Available Since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67513-plasmid-map-sequence-id-381535

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  19. GFP-BAZ1A

    Plasmid
    #65371
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Article
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Insert
    BAZ1A (BAZ1A Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    796I compared to all reference sequences
    Promoter
    CMV
    Available Since
    June 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

    Plasmid
    #154197
    Purpose
    Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    sgRNA-BC2-A, sgRNA-BC2-C
    Use
    Lentiviral; Catch barcode activation
    Expression
    Mammalian
    Promoter
    hU6 and mU6
    Available Since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154197-plasmid-map-sequence-id-308682

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Showing: 5541 - 5560 of 14239 results