We narrowed to 12,189 results for: LEA

Showing: 5541 - 5560 of 12189 results

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pEMS1532

Plasmid

#29259

Purpose

Expression of the reporter gene was not detected in the brain and eye using LacZ.

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple61 (DRD1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available since

Nov. 22, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1376

Plasmid

#29177

Purpose

Expression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple89 (GFAP Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1526

Plasmid

#29256

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple55 (DCX Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available since

Nov. 7, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1267

Plasmid

#29139

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple225 (TBR1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pBS-OMP-CreFNF

Plasmid

#15508

Depositor

Peter Mombaerts

Publication

Li et al Nature. 2004 Mar 25. 428(6981):393-9.

Insert

OMP targeting Vector with CRE FNF

Use

High copy number

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pTRIP-SFFV-EGFP-FLAG-UL36USP (C65)

Plasmid

#235549

Purpose

To express GFP-FLAG-UL36USP (C65) - region 1-520 of UL36

Depositor

Nicolas Manel

Publication

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

UL36USP (C65)

Use

Lentiviral

Tags

EGFP-FLAG

Mutation

N-terminal part of UL36 starting at the first ATG…

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pJRoC30_Th3.14

Plasmid

#188068

Purpose

FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)

Depositor

Sarel Fleishman

Publication

Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

Insert

FuncLib-designed high-redox potential laccase from Trametes hirsuta

Tags

S. cerevisiae evolved α-factor prepro-leader sign…

Expression

Yeast

Mutation

20 PROSS + 4 FuncLib mutations, described in publ…

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ERKsubDD-Venus-NES

Plasmid

#84630

Purpose

Encodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Publication

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ERKsubDD-Venus-NES

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-zic2a-NLS-BirA-2A-mCherry_Ras

Plasmid

#80065

Purpose

Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Publication

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Zic2a enhancer driving c-fos promoter

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eGFP-shAqp4-3

Plasmid

#223224

Purpose

mir30 based shRNA strategy, shRNA targeting mouse Aqp4

Depositor

Li-Huei Tsai

Publication

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shAqp4 (Aqp4 Mouse)

Use

AAV

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-5-enzymatic-domain

Plasmid

#214306

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Publication

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP5 (CASP5 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

aa133-434

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEF mito-cGFP (matrix)

Plasmid

#188904

Purpose

Expresses mitochondrial GFP

Depositor

Edwin Chapman

Publication

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

mitochondrial GFP

Tags

Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

Expression

Mammalian

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2alpha WT

Plasmid

#128812

Purpose

To make virus expressing Doc2alpha WT

Depositor

Edwin Chapman

Publication

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta WT

Plasmid

#128814

Purpose

To make virus expressing Doc2beta WT

Depositor

Edwin Chapman

Publication

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)

Plasmid

#191801

Purpose

Full length wt AtPCO4 with Nt-His tag

Depositor

Emily Flashman

Publication

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO4-1

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP-26sFur-B2AD-tevs-KKMP

Plasmid

#179644

Purpose

AND gate (TEVP/HCVP) SEAP RELEASE construct

Depositor

Xiaojing Gao

Publication

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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O-IL8-15

Plasmid

#166544

Purpose

scFv of a human scaffold targeting Interleukin-8. Antigen coverage aa 28-99 of 99, full-length excl. signal peptide

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, Sortag, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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V32M,L84M CoV-2 ORF8

Plasmid

#159934

Purpose

Protein overexpression in E.coli

Depositor

James Hurley

Publication

Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

CoV-2 ORF8 18-121 (ORF8 CoV-2)

Tags

6x Histidine, TEV cleavable

Expression

Bacterial

Mutation

V32M, L84M

Promoter

T7

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET30b GluBP600n

Plasmid

#119835

Purpose

Production of recombinant GluBP600n high affinity protein in E. coli

Depositor

Katalin Torok

Publication

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

GluBP600n

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BtuCD

Plasmid

#92208

Purpose

Expression of BtuCD in E. coli

Depositor

Douglas Rees

Publication

Locher et al Science. 2002 May 10;296(5570):1091-8.

Inserts

btuC (btuC )
btuD (btuD )

Tags

10x Histidine Tag and Enterokinase Cleavage Site

Expression

Bacterial

Promoter

T7 Promoter

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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