We narrowed to 19,002 results for: Tec

1. YPRCΔ15Ω2

   Plasmid

   #101709

   Purpose

   GoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. YPRCΔ15α2

   Plasmid

   #101711

   Purpose

   GoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pUPD-MAM33MTS (12)

   Plasmid

   #101693

   Purpose

   MAM33MTS in domestication vector

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Insert

   CCAT-[MTS from S. cerevisiae Superoxide dismutase [Mn] (YHR008C)from S. cerevisiae Mitochondrial acidic protein (YIL070C)]-AATG
   

   Use

   Yeast expression

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMB1-spect-PthrC3_25-eGFP

   Plasmid

   #107413

   Purpose

   This plasmid contain promoter PthrC3_25, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

   Depositor

   Kang Zhou

   Article

   Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   PthrC3_25

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMB1-spect-PthrC3_7-eGFP

   Plasmid

   #107412

   Purpose

   This plasmid contain promoter PthrC3_7, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

   Depositor

   Kang Zhou

   Article

   Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   PthrC3_7

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB6637

   Plasmid

   #106164

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6681 into Yarrowia lipolytica chromosomal location IntE_3, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b+R2(L171C)

   Plasmid

   #108239

   Purpose

   Expresses PdR-PCNA2(L171C) fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PdR-PCNA2(L171C) fusion protein
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   PCNA2,L171C

   Promoter

   T7 promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15T+1X

   Plasmid

   #108188

   Purpose

   PCNA1-PdX fusion protein

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PCNA1-PdX fusion protein
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   C73S/C85S (PdX)

   Promoter

   T7 promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLENTICRISPR sgNFS1

   Plasmid

   #102979

   Purpose

   Cutting NFS1 genomic locus

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   sgNFS1 (NFS1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-NMBR-eLOV-TEVcs-FLAG-GAL4-V5

    Plasmid

    #104843

    Purpose

    b2AR-SPARK TF component

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    NMBR-eLOV-TEVcs-FLAG-GAL4-V5
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. YIp128-P30-His-pol30(K127/164R)

    Plasmid

    #99545

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; Leu2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K127R, K164R

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA

    Plasmid

    #87572

    Purpose

    PV4 gRNA entry vector for CRISPR decoder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA

    Plasmid

    #89056

    Purpose

    PV3 gRNA entry vector for CRISPR decoder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3_zfh1_CP-candidate_luc+

    Plasmid

    #86391

    Purpose

    Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer
    

    Use

    Luciferase

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pILGFPB6

    Plasmid

    #83557

    Purpose

    Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL10 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. const #7-RFP

    Plasmid

    #81081

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter - BBa_J23109

    Available Since

    Aug. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pconst #6-RFP

    Plasmid

    #81080

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter - BBa_J23117

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. shCDK4(1)

    Plasmid

    #73550

    Purpose

    shRNA(1) against CDK4

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

19. shCDK4(2)

    Plasmid

    #73551

    Purpose

    shRNA(2) against CDK4

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pCellFree_G03 TCEB3B

    Plasmid

    #67117

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCEB3B (ELOA2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only