We narrowed to 15,941 results for: genome

Showing: 5561 - 5580 of 15941 results

Results per page:

Sort results by:

pGM202T7

Plasmid

#69993

Purpose

E. coli/Streptomyces expression vector containing the T7-promoter

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aphII
tsr
rep
sso
T7 promoter
dso
His-Orf

Tags

N-terminal and C-terminal His tag encoding sequen…

Expression

Bacterial

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
eGFPbait-E2A-KalTA4-pA donor vector

Plasmid

#61069

Purpose

for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

Depositor

Filippo Del Bene

Article

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Inserts

eGFPbait
KalTA4

Use

Zebrafish expression

Tags

E2A

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pKMD106e - intein-Cas9(S219)

Plasmid

#64190

Purpose

Expresses intein-Cas9(S219) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-BE-VQRCas9

Plasmid

#101740

Purpose

expression of an optimized Cas9 for base-editing in zebrafish

Depositor

Shuo Lin

Article

Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

Insert

rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS

Use

CRISPR

Expression

Mammalian

Mutation

D10A, VQR mutations

Promoter

CMV

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_TOP2B

Plasmid

#183325

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP2B gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP2B

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_TOP2A

Plasmid

#183324

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP2A gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP2A

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI Cas9 H2B-RFP

Plasmid

#92397

Purpose

CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFnCas12a_NT

Plasmid

#169838

Purpose

Expresses FnCas12a and guide RNA in Bacteria

Depositor

John van der Oost

Article

Mohanraju et al CRISPR J. 2021 Feb;4(1):82-91. doi: 10.1

Insert

FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

pXynA

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_FGFR3

Plasmid

#183291

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR3

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR-GFPuniv

Plasmid

#46085

Purpose

In vivo recombination assay fluorescent reporter

Depositor

Raymond Monnat

Article

Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

Insert

DR-GFPuniv reporter

Use

Recombination reporter plasmid

Expression

Mammalian

Mutation

E5G and K163R mutations in EGFP relative to wild-…

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pJIT163-2NLSCas9

Plasmid

#53064

Purpose

Expression of rice codon-optimized Cas9 in plant cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

Cas9

Use

Plant expression

Mutation

Rice codon optimized

Promoter

2x35S

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
AsdCas12a VPR

Plasmid

#188512

Purpose

Expresses FLAG tagged AsdCas12a VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D908A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-STMN1

Plasmid

#31326

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

stathmin 1/oncoprotein 18 (STMN1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2796

Plasmid

#63641

Purpose

pCfB2796 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR1319

Plasmid

#111116

Purpose

Expresses UBE2T_+_202311076.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

UBE2T gRNA (UBE2T Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcU6_1 sgRNA

Plasmid

#92395

Purpose

Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

chick U6.1 promoter and gRNA cloning cassette

Use

CRISPR

Expression

Mammalian

Promoter

chick U6.1

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CD200RCD4d3+4-bio-linker-His

Plasmid

#36153

Purpose

A positive control receptor bait with bio-linker-His (rat CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs plasmid

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200R (Cd200r1 Rat)

Tags

His tag, biotinylation peptide, and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGK61

Plasmid

#228121

Purpose

Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells

Expression

Mammalian

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pL-SFFV.Reporter.RFP657.PAC

Plasmid

#61395

Purpose

Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEP300-pAAV-CMV-MCS2-pA

Plasmid

#112138

Purpose

CMV driven Multi Cloning Site-2

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge