We narrowed to 1,622 results for: nbas

1. WT FL USP7

   Plasmid

   #227214

   Purpose

   Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7-promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOET5 GII.4 Sydney noro-VLP

   Plasmid

   #218092

   Purpose

   Forms Sydney GII.4 Sydney norovirus-like particle in insect cells by expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank ID: AFV08795).

   Depositor

   Minna Hankaniemi

   Insert

   noro-VLP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJYDN3

   Plasmid

   #162456

   Purpose

   Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Type

   Empty backbone

   Use

   Shuttle vector bacteria/yeast

   Tags

   ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MARS

   Plasmid

   #205232

   Purpose

   Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells

   Depositor

   Jeremy Baskin

   Insert

   PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
   

   Use

   Tags

   Nuclear Export Sequence and mScarlet-i

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKG113

   Plasmid

   #63073

   Purpose

   Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   CENP-T (CENPT Human)
   

   Use

   Retroviral

   Tags

   EGFP and TEV-S

   Expression

   Mammalian

   Mutation

   E. coli codon optimized. G217R mutation compared …

   Promoter

   Available since

   Jan. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOX + kqt3

   Plasmid

   #16138

   Depositor

   Larry Salkoff

   Article

   Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

   Insert

   Caenorhabditis elegans potassium channel (kqt-3 Nematode)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   lacks aa1-16 compared to GenBank reference AY5729…

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pKG204

   Plasmid

   #63078

   Purpose

   Retroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   HP1 alpha (CBX5 Human)
   

   Use

   Retroviral

   Tags

   EGFP and TEV-S

   Expression

   Mammalian

   Mutation

   V151I mutation compared to GenBank reference sequ…

   Promoter

   Available since

   Jan. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLP-TRE2-hSox2

   Plasmid

   #49392

   Purpose

   inducible (dox) expression of hSox2

   Depositor

   Randall McKinnon

   Insert

   Sox2 (SOX2 Human)
   

   Use

   Cre/Lox; Donor vector

   Tags

   6xHN

   Expression

   Mammalian

   Mutation

   TGA (stop) to CCA (Pro) at nt 1027 with respect t…

   Promoter

   Tet-responsive

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

9. PROM5_Ntprrn_rev_chlo

   Plasmid

   #163952

   Purpose

   L0 part - promoter

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   from GenBank: KT364744.3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. PROM5_Ntpsba_chlo

    Plasmid

    #163953

    Purpose

    L0 part - promoter

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    from GenBank: KT364744.3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pSM2

    Plasmid

    #85753

    Purpose

    pDONR split marker vector (GenBank accession number: KX904529) for fungal gene deletion. Used with pSM1.

    Depositor

    Damien Fleetwood

    Article

    Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSM1

    Plasmid

    #85752

    Purpose

    pDONR split marker vector (GenBank accession number: KX904528) for fungal gene deletion. Used with pSM2.

    Depositor

    Damien Fleetwood

    Article

    Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMZ036

    Plasmid

    #66973

    Purpose

    Expresses zebrafish Chk1

    Depositor

    Michael Lampson

    Article

    Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

    Insert

    zebrafish Chk1
    

    Use

    Zebrafish mrna injection

    Tags

    Expression

    Mammalian

    Mutation

    Q341H mutation compared to GenBank reference sequ…

    Promoter

    CMV

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMUH_unc84_2XGFP

    Plasmid

    #46023

    Depositor

    Sean Eddy

    Article

    Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

    Insert

    UNC-84 (unc-84 )
    

    Use

    10xuas-phi31-expression vector

    Tags

    2XGFP

    Expression

    Mutation

    Two amino acid mutations (F279L and L500P) compar…

    Promoter

    10XUAS

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EMTB-mCherry

    Plasmid

    #26742

    Depositor

    William Bement

    Article

    Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

    Insert

    ensconsin (MAP7 Human)
    

    Use

    Probe for microtubules

    Tags

    2x mCherry and Myc

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TRF2-IRESS-eGFP

    Plasmid

    #19798

    Depositor

    Mark Mattson

    Article

    van Steensel et al Cell. 1998 Feb 6. 92(3):401-13.

    Insert

    TRF2 (TERF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    When compared to GenBank reference sequence NP_00…

    Promoter

    CMV-F

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

17. 2xMARS

    Plasmid

    #205233

    Purpose

    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    

    Use

    Tags

    Nuclear Export Sequence and mScarlet-i

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC-hrDNA-12.0

    Plasmid

    #206320

    Purpose

    A plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)

    Depositor

    Brian McStay

    Article

    van Sluis et al Methods Mol Biol. 2016;1455:3-14. doi: 1

    Insert

    Intergenic spacer of human rDNA repeat
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET51b(+)_ALFA-tag-SNAP

    Plasmid

    #136628

    Purpose

    Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 Rod Opsin

    Plasmid

    #109361

    Purpose

    Human rod opsin

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin (RHO Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLPS-hSox2-gfp

    Plasmid

    #49390

    Purpose

    mammalian expression of human Sox2 fused to EGFP

    Depositor

    Randall McKinnon

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    TGA (stop) to CCA (Pro) at nt 1027 with respect t…

    Promoter

    CMV

    Available since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDUP3

    Plasmid

    #92174

    Purpose

    Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.

    Depositor

    Judith Berman

    Article

    Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

    Insert

    NAT1
    

    Use

    Genbank: u03440.1

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKHR7

    Plasmid

    #74624

    Purpose

    a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.

    Depositor

    David Grunwald

    Article

    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

    Insert

    cmlc2::mCherry
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    cmlc2

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMUH_unc84_tdTFlag

    Plasmid

    #46024

    Depositor

    Sean Eddy

    Article

    Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

    Insert

    UNC-84 (unc-84 )
    

    Use

    10xuas-phi31-expression vector

    Tags

    tdTomato

    Expression

    Mutation

    Two amino acid mutations (F279L and L500P) compar…

    Promoter

    10XUAS

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAc5.1B-EGFP-DmFMR1_B

    Plasmid

    #145920

    Purpose

    Insect Expression of DmFMR1

    Depositor

    Elisa Izaurralde

    Insert

    DmFMR1 (Fmr1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two amino acid mutations (T214I and N501S) and th…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 3TERM_bba_b0015_rev_chlo

    Plasmid

    #163931

    Purpose

    L0 part - terminator

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    from GenBank: KT364744.3, prokaryotic double terminator BBa_B0015
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pAc5.1B-lambdaN-HA-DmFMR1_B

    Plasmid

    #145940

    Purpose

    Insect Expression of DmFMR1

    Depositor

    Elisa Izaurralde

    Insert

    DmFMR1 (Fmr1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two amino acid mutations (N45D and G558R) and thr…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CyclinB-GFP

    Plasmid

    #54465

    Purpose

    CyclinB-GFP in pIVT vector for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

    Insert

    CyclinB
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K67E and I150T mutations compared to GenBank refe…

    Promoter

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

29. DHRS4

    Plasmid

    #39071

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DHRS4 (DHRS4 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pLPS-hAkt1-gfp

    Plasmid

    #49388

    Purpose

    mammalian expression of human Akt1 fused to EGFP

    Depositor

    Randall McKinnon

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    tga to cca at nt 1725 with respect to GenBank ID …

    Promoter

    CMV

    Available since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

31. pDNR-hSox2

    Plasmid

    #49389

    Purpose

    donor vector for cloning human Sox2 with 6xHN tag

    Depositor

    Randall McKinnon

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Cre/Lox; Donor vector

    Tags

    6xHN (6 repeating His-Asn subunits)

    Expression

    Mutation

    TGA (stop) to CCA (Pro) at nt 1027 with respect t…

    Promoter

    none

    Available since

    Nov. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

32. pDNR-hAkt1

    Plasmid

    #49387

    Purpose

    donor vector for cloning human Akt1 with 6xHN tag

    Depositor

    Randall McKinnon

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Cre/Lox; Donor vector

    Tags

    6xHN (6 repeating His-Asn subunits)

    Expression

    Mutation

    tga to cca at nt 1725 with respect to GenBank ID …

    Promoter

    none

    Available since

    Dec. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

33. G392A FL USP7

    Plasmid

    #227217

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392A

    Promoter

    T7-promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. N418S FL USP7

    Plasmid

    #227215

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    N418S

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. G392D FL USP7

    Plasmid

    #227216

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392D

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. K420E FL USP7

    Plasmid

    #227223

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    K420E

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. L373F FL USP7

    Plasmid

    #227220

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L373F

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Y331H FL USP7

    Plasmid

    #227218

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Y331H

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. P273H FL USP7

    Plasmid

    #227221

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    P273H

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. M225I FL USP7

    Plasmid

    #227219

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    M225I

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge