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pHR: pPGK PYL1-sfGFP-NLS

Plasmid

#174116

Purpose

Expression of PYL1-sfGFP-NLS

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

PYL1-sfGFP-NLS

Use

Lentiviral

Promoter

PGK

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAB147

Plasmid

#101662

Purpose

Opto-T7RNAP*(302)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(302)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p214_LTJ_1kbCD90.1Template

Plasmid

#82667

Purpose

1kb HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

1kb genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-EMX1

Plasmid

#140581

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA EMX1

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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B2329_mCherry-FKBP2x

Plasmid

#215103

Purpose

Expresses mCherry-FKBP-FKBP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

mCherry-FKBP-FKBP

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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c7_HLL

Plasmid

#63125

Purpose

cysteine free Mm-cpn mutant

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_HLL (NEWENTRY )

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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p229_LTJ_2kbCD90.1Template

Plasmid

#82668

Purpose

2kb HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

2kb genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pXCX.CMV.EGFP

Plasmid

#38053

Depositor

James Uney

Article

Glover et al Mol Ther. 2002 May;5(5 Pt 1):509-16.

Insert

EGFP

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pICXC3H

Plasmid

#49022

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Promoter

AOX1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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spoT_NS1

Plasmid

#100914

Purpose

expresses spoT (from native promoter) from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

synpcc7942_1377 (spoT)

Use

Cyanobacteria cloning vector

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAL198

Plasmid

#22281

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

p!SV40-mCherry-WaspGBD

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pYEXC3GH

Plasmid

#49027

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSYC-102

Plasmid

#74790

Purpose

pCS4+-NLS-EGFP-T2A-mCherry-CAAX

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

NLS-EGFP-T2A-mCherry-CAAX

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-Dr-TrkB

Plasmid

#127964

Purpose

Myr mCherryDrTrkB expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

Myr-mCherry-3xSAG-Dr-hel-4- TrkB

Expression

Mammalian

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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p248_LTJ_2kbCD45.1Template

Plasmid

#82662

Purpose

2kb HDR template for murine CD45.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

2kb genomic DNA sequence from murine CD45.1

Use

Hdr template for gene editing

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDS282

Plasmid

#34983

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

cpPDZ–mCherry

Expression

Yeast

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pNC-KSE401

Plasmid

#193435

Purpose

Nimble cloning-compatible vector for genome editing in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Palladin-C-7

Plasmid

#55318

Purpose

Localization: Cytoskeleton, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Palladin

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pXCX.CMV.EGFP.WPRE

Plasmid

#38055

Depositor

James Uney

Article

Glover et al Mol Ther. 2002 May;5(5 Pt 1):509-16.

Insert

EGFP

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCZGY2839

Plasmid

#87765

Purpose

Prgef-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Prgef-1-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Prgef-1

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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