We narrowed to 36,400 results for: ACE
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Plasmid#140014PurposePlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianMutationY126A, Y197FPromoterEF1Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgRNA
Plasmid#124844PurposeVector for Cre-dependent expression of SaCas9DepositorInsertSaCas9, gRNA scaffold
UseAAV, CRISPR, and Mouse TargetingTagsNLS and NLS-3xHAAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AMPKAR
Plasmid#192446PurposeGenetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.DepositorInsertExRai-AMPKAR
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianPromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEx-ER-TurboID
Plasmid#160857PurposeCre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorHas ServiceAAV8InsertER TurboID
UseAAVTagsHA and V5PromoterCAGAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-RpHLuorin2
Plasmid#171720PurposeLysosomal expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
Tagsratiometric pHluorin2ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AMPKAR(T/A)
Plasmid#192447PurposeNon-phosphorylatable negative-control mutant of ExRai-AMPKAR.DepositorInsertExRai-AMPKAR(T/A)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationThr 6 in AMPK substrate mutated to Ala.PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-tMCK-ER-TurboID
Plasmid#149412PurposeMuscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertER-TurboID
UseAAVTagsHA and V5PromotertMCKAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-ExRai-AMPKAR
Plasmid#192450PurposeExRai-AMPKAR targeted to the nucleus.DepositorInsertExRai-AMPKAR-NLS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK017 pDEST exorh:EGFP
Plasmid#195983PurposeTransgenesis backboneDepositorTypeEmpty backboneUseOtherAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Hygro_GCaMP6s
Plasmid#164591PurposeStable integration and expression of GCaMP6s in mammalian cellsDepositorInsertGCaMP6s
UseLentiviralTags6xHisExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK3_038
Plasmid#123752PurposeEncodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK systemDepositorInsertR-FlincA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterNative promoter from pMUT2 relB/relE operon and P…Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpRY Cas9
Plasmid#188491PurposeExpresses FLAG tagged SpRY Cas9DepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST exorh:mCherry
Plasmid#195989PurposeTransgenesis backboneDepositorTypeEmpty backboneUseOtherAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTnsABC
Plasmid#160031PurposeExpresses V. cholerae TnsABCDepositorInsertVchTnsA, VchTnsB, VchTnsC
UseCRISPR; TransposonExpressionBacterialMutationnoAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRXRα
Plasmid#135910PurposeHuman Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 PromoterDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-synPCB2.1
Plasmid#139478PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
ExpressionMammalianPromoterCAG promoterAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TBG-ER-TurboID
Plasmid#149415PurposeHepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorHas ServiceAAV8InsertER-TurboID
UseAAVTagsHA and V5PromoterTBGAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Emerin pEGFP-C1 (637)
Plasmid#61993Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4.20-HIF1A prom
Plasmid#40173DepositorInsertHIF1A promoter fragment (HIF1A Human)
ExpressionMammalianAvailable SinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro_G-CEPIA1er
Plasmid#164590PurposeStable integration and expression of G-CEPIA1er in mammalian cellsDepositorInsertG-CEPIA1er
UseLentiviralTagsMycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mito-ExRai-AMPKAR
Plasmid#192448PurposeExRai-AMPKAR targeted to the mitochondrial outer membrane.DepositorInsertMito-ExRai-AMPKAR
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dInPAkt
Plasmid#181834PurposeGenetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).DepositorInsertLyn-dInPAkt
TagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianPromoterCMVAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mem-TurboID
Plasmid#149409PurposeMammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selectionDepositorInsertmem-TurboID
TagsHA and V5ExpressionMammalianPromoterCMVAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK054 pDEST exorh:mCerulean
Plasmid#195985PurposeTransgenesis backboneDepositorTypeEmpty backboneUseOtherAvailable SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2Apuro-TRE-synPCB2.0
Plasmid#139481PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
UseTol2 transposon donor vectorExpressionMammalianPromoterTet responsive elementAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Emerin pEGFP-N2 (588)
Plasmid#61985Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
amilCP chromoprotein
Plasmid#117847PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP
UseSynthetic Biology; Escherichia coliAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
PLX306 iCre tetO tomato rtTA
Plasmid#158050Purposelenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomatoDepositorInserttdTomato
TagsnoneExpressionMammalianPromotertetO promoterAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZW6 (CK2alpha)
Plasmid#27086DepositorAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Tv9nL
Plasmid#188069PurposePROSS-designed high-redox potential laccase from Trametes versicolorDepositorInsertPROSS-designed high-redox potential laccase from Trametes versicolor
TagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation79 PROSS mutations, described in publicationAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGAselect
Plasmid#195714PurposepGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceFeb. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
SpRY dCas9 VPR
Plasmid#188511PurposeExpresses FLAG tagged SpRY dCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralExpressionMammalianMutationD10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…PromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Padron2
Plasmid#172355PurposeExpresses the reversibly switchable fluorescent protein Padron2 in bacterial cellsDepositorInsertPadron2
Tags6xHisTag (N terminal on backbone)ExpressionBacterialPromoteraraBADAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB46 (CK2beta)
Plasmid#27085DepositorAvailable SinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR
Plasmid#154827PurposeLanding pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)DepositorInsertsTagBFP
HygR
ZsGreen1-DR
UseCRISPR and Cre/LoxTagsPESTExpressionMammalianPromoterCMV, EF1a, and SV40 early promoterAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB1 (CK2alpha)
Plasmid#27083DepositorAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPathBrick
Plasmid#65006PurposeE. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricksDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive native promotersAvailable SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_nat
Plasmid#184918PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only