We narrowed to 81,643 results for: TRI

1. single polypeptide FPX biosensor for both caspase-3 and -8

   Plasmid

   #60886

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for caspase-3 and 8
   

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pLKO.1 shJUND puro

   Plasmid

   #58698

   Purpose

   Lentiviral shRNA vector for knockdown of human JUND

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   shJUND (JUND Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pPKm-248

   Plasmid

   #90504

   Purpose

   Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tPCYA - IRES - MTS - tHO1 - P2A - MTS - tFD - P2A - MTS - tFNR

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
   

   Use

   Lentiviral

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ntng1.a-AP-His

   Plasmid

   #71984

   Purpose

   Expresses the extracellular region of the Netrin G1, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ntng1.a (Ntng1 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mScarlet-gamma9

   Plasmid

   #140097

   Purpose

   Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   GNGT2
   

   Tags

   N-terminal mScarlet tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX 4T1 RARalpha

   Plasmid

   #35557

   Depositor

   Jonathan Kurie, Harish Srinivas

   Article

   Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

   Insert

   RARalpha (RARA Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

7. LentiCas9-sgCh2-2-Blast

   Plasmid

   #199645

   Purpose

   Expresses Cas9 and sgRNA control guide targeting intergenic region

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A
   

   Use

   Lentiviral

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mcherry Gamma 9 ---pcDNA3.1

   Plasmid

   #64203

   Purpose

   G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

   Depositor

   Narasimhan Gautam

   Article

   Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   G protein Gamma 9 (GNGT2 Human)
   

   Tags

   6xHis and mcherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pEGFP-C3-PLK4-S305A-3xFLAG

   Plasmid

   #69839

   Purpose

   This plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

   Insert

   PLK4 (PLK4 Human)
   

   Tags

   3xFLAG tag and EGFP

   Expression

   Mammalian

   Mutation

   S305A

   Promoter

   CMV

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPN062

    Plasmid

    #91595

    Purpose

    Express sgRNA targeting human DFNA5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DFNA5 (GSDME Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPN063

    Plasmid

    #91596

    Purpose

    Express sgRNA targeting human DFNA5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DFNA5 (GSDME Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pScalps_Puro_mCebpe

    Plasmid

    #79076

    Purpose

    Expression of mouse Cebpe

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Cebp epsilon (Cebpe Mouse)
    

    Use

    Lentiviral

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pRSET-pr-mKikGR1 (mKikGR1-V69T)

    Plasmid

    #99227

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mKikGR1
    

    Expression

    Bacterial

    Mutation

    monomeric variant with mutation V69T (Eos notatio…

    Promoter

    T7

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-sgRELA

    Plasmid

    #83944

    Purpose

    Lentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgRELA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFSynW Substance P-pHluorin IRES mRuby3

    Plasmid

    #195699

    Purpose

    Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Tac1 (Tac1 Mouse)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSUPER-hHSF2 shRNA

    Plasmid

    #118346

    Purpose

    This plasmid expresses shRNA against hHSF2.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF2 shRNA (HSF2 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-GFP-EBA175-Flag

    Plasmid

    #154970

    Purpose

    Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Tags

    GFP and signal peptide (residues 1 to 32) from TG…

    Expression

    Mammalian

    Mutation

    insert codes for EBA175 residues 1284 to 1462

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-hs-3xHA-delta6xNHL-LIN-41

    Plasmid

    #52722

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 593–868

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. MDH1-shSHP2-3-H2Kb2

    Plasmid

    #17854

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    shSHP2 (PTPN11 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBiex PKC - Peptide 8 mCit mCer

    Plasmid

    #83563

    Purpose

    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

    Insert

    PRKCA (PRKCA Human)
    

    Tags

    10 nm ER/K Helix, FLAG purification tag, TEV Prot…

    Expression

    Bacterial and Insect

    Mutation

    Only catalytic domain of PKC (aa 335-672)

    Promoter

    T7 Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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