We narrowed to 2,423 results for: LRE

1. pAG416GAL-EGFP

   Plasmid

   #166140

   Purpose

   EGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

   Plasmid

   #122084

   Purpose

   Express M.EcoGII fused to TRF1 with detabilization domain - inducible

   Depositor

   Laure Crabbe

   Article

   Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

   Insert

   DD-linker-M.EcoGII-V5-TERF1
   

   Use

   Retroviral

   Tags

   V5 tag on Nter of TERF1

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLv-EF1a-Parkin-A92mKO2

   Plasmid

   #213559

   Purpose

   Lentiviral vector for stable expression of Parkin, internally tagged with mKO2

   Depositor

   Lani Wu, Steven Altschuler

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAVS1-shRNA

   Plasmid

   #82697

   Purpose

   For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.

   Depositor

   Alan Mullen

   Article

   Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

   Type

   Empty backbone

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   hPGK, U6

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-Hygro-EF1A-OB-Linker-eGFP

   Plasmid

   #176068

   Purpose

   EGFP fused to the C-terminus of an OB domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   OB
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-FLEX-SaCas9-U6-sgGabrg2

   Plasmid

   #124857

   Purpose

   Mutagenesis of Gabrg2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg2 (Gabrg2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFPC1-ORAI1-EC-HA

   Plasmid

   #174335

   Purpose

   encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   human Orai1 (ORAI1 Human)
   

   Use

   Tags

   GFP in the N-terminus and HA tag in the extracell…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGAMA-YAP

   Plasmid

   #74942

   Purpose

   Lentiviral over-expression vector for YAP

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-mCherry

   Expression

   Mutation

   Promoter

   EF1α

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pXJ-His-VSIR

   Plasmid

   #232663

   Purpose

   transient expresses VSIR in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   VSIR (C10orf54 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-Hygro-EF1A-FHA-Linker-eGFP

    Plasmid

    #176065

    Purpose

    EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    FHA
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PGK EGFP-PPM1H-WT

    Plasmid

    #200084

    Purpose

    Mammalian expression of wild-type PPM1H

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV5-25BoxB

    Plasmid

    #60817

    Purpose

    Cloning vector, 25 repeats of 19 bp BoxB sequence from bacteriophage-λ, for RNA labeling. BoxB-RNA is bound by 22 amino acid RNA binding domain of λ-anti-terminator protein N, e.g. tagged with GFP

    Depositor

    Maria Carmo-Fonseca

    Article

    Martin et al Cell Rep. 2013 Sep 26;4(6):1144-55. doi:

    Insert

    25x BoxB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    25 repeats of BoxB sequence

    Promoter

    CMV

    Available since

    Nov. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ER-mGreenLantern

    Plasmid

    #164463

    Purpose

    Localization and retention of mGreenLantern fluorescent protein in the ER

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eGFP-KIF1A-Rigor

    Plasmid

    #172207

    Purpose

    Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF1A (Kif1a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 251 to Alanine

    Promoter

    CMV

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-VTKS2-TVA-eGFP-N2cG

    Plasmid

    #175439

    Purpose

    Cre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

    Depositor

    Gordon Fishell

    Article

    Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

    Insert

    TVA-P2A-eGFP-P2A-N2cG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CLYBL-TO-PAX7

    Plasmid

    #204346

    Purpose

    Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    human PAX7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-Hygro-EF1A-Macro-Linker-eGFP

    Plasmid

    #176067

    Purpose

    EGFP fused to the C-terminus of a Macrodomain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    Macro
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-SUMO-mGSDMD

    Plasmid

    #111560

    Purpose

    Express mouse GSDMD in E.coli

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Tags

    His, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-Hygro-EF1A-WWE-Linker-eGFP

    Plasmid

    #176072

    Purpose

    EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    WWE
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR LAT/Zap70 iLID-Drop

    Plasmid

    #171038

    Purpose

    Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-labeled.

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

    Insert

    LAT (LAT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-Hygro-EF1A-PBZC-Linker-eGFP

    Plasmid

    #176070

    Purpose

    EGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PBZ-C
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV-Hygro-EF1A-PBM-Linker-eGFP

    Plasmid

    #176069

    Purpose

    EGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PBM
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre

    Plasmid

    #134308

    Purpose

    Expresses Synaptophysin-TdTomato and also tamoxifen dependant Cre (OHT-Cre, aka Cre-ER)

    Depositor

    Elly Nedivi

    Article

    Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

    Inserts

    Synaptophysin (Syp Mouse, Rat)
    Cre-ER
    

    Use

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcmv3-IRG1-L272Q-HA

    Plasmid

    #198184

    Purpose

    Mammalian expression of HA-tagged human ACOD1 (IRG1) L272Q

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    ACOD1 (ACOD1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The leucine in IRG1 at position 272 mutates to gl…

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 1000.pCCLsin.cPPT.hPGK.Ascl1.WPRE

    Plasmid

    #67291

    Purpose

    Induce human iN conversion from fibroblast

    Depositor

    Malin Parmar

    Article

    Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

    Insert

    Asc1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PGK ARF6(Q67L)-CFP

    Plasmid

    #200428

    Purpose

    Mammalian expression of Q67L mutation in ARF6

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    PGK

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-Hygro-EF1A-LivePARBackbone

    Plasmid

    #176526

    Purpose

    Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBABR IRFP-Bcd-LEXY

    Plasmid

    #182595

    Purpose

    Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    Bcd (bcd Fly)
    

    Use

    Tags

    IRFP and LEXY

    Expression

    Insect

    Mutation

    Promoter

    maternal tubulin

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. psigma2-EGFP

    Plasmid

    #53610

    Purpose

    Expresses sigma2 of adaptor protein of AP2

    Depositor

    Tom Kirchhausen

    Article

    Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

    Insert

    sigma2 (Ap2s1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA 3.3 hFZD4

    Plasmid

    #115908

    Purpose

    Expresses human FZD4 in mammalian cells.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    FZD4 (FZD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDEST40-2XHA-Src-K298M

    Plasmid

    #140314

    Purpose

    Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA4/TO/GFP-ZNF598

    Plasmid

    #141191

    Purpose

    Expresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-TORCAR-NLS

    Plasmid

    #64931

    Purpose

    Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR-NLS (EIF4EBP1 Human, Synthetic)
    

    Use

    Tags

    Cerulean, Nuclear localization signal (NLS), and …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAG426GAL-EGFP

    Plasmid

    #166143

    Purpose

    EGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAG426GAL-mRuby3

    Plasmid

    #166144

    Purpose

    mRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDEST40-2XFL-Src-S4-KD

    Plasmid

    #140297

    Purpose

    Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    contains only the SH4 and KD regions

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. canxTM-mEmerald (cytosolic)

    Plasmid

    #186962

    Purpose

    Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CANX (CANX Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Only express calnexin (482-end) and add ER signal…

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA4TO-3XFLAG Halo WT nsp1

    Plasmid

    #175412

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1 (ORF1ab CoV2)
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH

    Plasmid

    #74289

    Purpose

    pAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    H2B-GFP and oG (optimized Glycoprotein)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    Ef1a

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMSCV-NLS-CBGreen-FIRE-puro

    Plasmid

    #139195

    Purpose

    Luminescent reporter of ERK activity

    Depositor

    Matthew Lazzara

    Article

    Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

    Insert

    CBG99
    

    Use

    Luciferase and Retroviral

    Tags

    Fra1 PEST domain fragment (FIRE) and nuclear loca…

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDEST40-2XFL-Src-K298M

    Plasmid

    #140315

    Purpose

    Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid

    #139086

    Purpose

    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. pCAG-Bmpr2Δ751-813aa

    Plasmid

    #163603

    Purpose

    Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The LIMK-binding region (751-813 aa) is deleted

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pαH-S-RRAR

    Plasmid

    #164569

    Purpose

    SARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-RRAR variant)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pcDNA5/FRT/TO-Intron-CterFlag-MePCE

    Plasmid

    #113549

    Purpose

    Mammalian expression plasmid that can be used with the Flp-In T-REx system.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    MePCE (MEPCE Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

    Plasmid

    #184635

    Purpose

    Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA3.1-STIM1-Venus-173-C

    Plasmid

    #87619

    Purpose

    Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    STIM1-Venus173-C (STIM1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pDEST40-2XHA-Src-Y419F

    Plasmid

    #140316

    Purpose

    Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Y419F

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCMV-BEACON2

    Plasmid

    #171698

    Purpose

    Expresses BEACON2 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

    Insert

    BEACON2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_W98Y/W104A/Y130F

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

    Plasmid

    #101300

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-509, 670-698, and 699-906 …

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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