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167,120 results

Showing: 11881 - 11920 of 167120 results

  2. sUPRa

    Plasmid
    #223242
    Purpose
    sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels
    Depositor
    Colin Akerman
    Article
    Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10
    Insert
    sUPRa
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Custom
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    223242-plasmid-map-sequence-id-450772

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  6. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

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  7. pInducer10b-EGFP-KRAS G12V

    Plasmid
    #164925
    Purpose
    Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells
    Depositor
    Ji Luo
    Article
    Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:
    Inserts
    Enhanced green fluorescent protein
    KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Glycine 12 changed to valine (G->T 3156)
    Promoter
    Ubc Promoter and Ubc promoter
    Available Since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164925-plasmid-map-sequence-id-333648

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  10. pIS0

    Plasmid
    #12178
    Purpose
    3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production
    Depositor
    David Bartel
    Article
    Yekta et al Science. 2004 Apr 23. 304(5670):594-6.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12178-plasmid-map-sequence-id-181181

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  16. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid
    #139996
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139996-plasmid-map-sequence-id-275405

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  18. pET-Trx-mSA2

    Plasmid
    #52320
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Use
    Tags
    FLAG, His, S tag, TEV site, and Thioredoxin
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52320-plasmid-map-sequence-id-84861

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  22. pEBB-SBP-HA

    Plasmid
    #36100
    Depositor
    Ezra Burstein
    Type
    Empty backbone
    Use
    Tags
    HA and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    36100-plasmid-map-sequence-id-44719

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  23. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43861-plasmid-map-sequence-id-59200

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  29. pVITRO1-M80-F2-IgG1/κ

    Plasmid
    #50383
    Purpose
    Expresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chain
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    human Gamma 1 heavy chain expression cassette with unknown specificity
    human Kappa light chain expression cassette with unknown specificity
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available Since
    Jan. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    50383-plasmid-map-sequence-id-75289

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  30. mEmerald-Alpha-V-Integrin-25

    Plasmid
    #53985
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Alpha-V Integrin (ITGAV Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    53985-plasmid-map-sequence-id-257384

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  36. sgRNA_expression_vector

    Plasmid
    #210212
    Purpose
    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.
    Depositor
    Albert Jeltsch
    Article
    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    U6
    Available Since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    210212-plasmid-map-sequence-id-416400

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  38. pDONR221-SLC16A1_STOP

    Plasmid
    #161100
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC16A1 (SLC16A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161100-plasmid-map-sequence-id-359436

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Showing: 11881 - 11920 of 167120 results