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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 59,693 results for: %s

Showing: 41 - 60 of 59693 results

  1. GATA3-pCDNA3.1

    Plasmid
    #115271
    Purpose
    Expresses human GATA3 in mammalian cells
    Depositor
    Arne S. Schaefer
    Insert
    GATA3 (GATA3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115271-plasmid-map-sequence-id-220672

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  3. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  9. pDual-eGFP

    Plasmid
    #63215
    Purpose
    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pSG5-BRLF1

    Plasmid
    #72635
    Purpose
    Mammalian expression vector for Epstein-Barr virus Rta immediate-early replication and transcription activator
    Depositor
    S. Diane Hayward
    Article
    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.
    Insert
    BRLF1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. pAF123

    Plasmid
    #135921
    Purpose
    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    135921-plasmid-map-sequence-id-270083

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  13. pSG5-BMLF1

    Plasmid
    #72636
    Purpose
    Mammalian expression vector for Epstein-Barr virus Mta immediate-early post-transcriptional regulator
    Depositor
    S. Diane Hayward
    Article
    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.
    Insert
    BMLF1/BSLF2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Q157H
    Promoter
    Available Since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. pDual-eGFP(Y203)

    Plasmid
    #63559
    Purpose
    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Y203)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed threonine at position 203 with respect to…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pAF92

    Plasmid
    #135929
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    mCherry fused to TNFSF9
    Use
    Transposon
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135929-plasmid-map-sequence-id-270167

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Showing: 41 - 60 of 59693 results