We narrowed to 7,372 results for: tet

1. PB-TET

   Plasmid

   #20909

   Purpose

   piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   Gateway Destination Vector
   

   Use

   piggybac

   Tags

   IRES-bGeo

   Expression

   Mammalian

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. tet-pLKO.puro_shGFP

   Plasmid

   #110421

   Purpose

   shGFP control, silence GFP gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GFP
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1/TO (RNA PolIII)

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGDP4 tet(A)

   Plasmid

   #112887

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   tet(A)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Sequence was codon optimized for E. coli K12 usin…

   Promoter

   Plac

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. tet-pLKO.puro_shLuc

   Plasmid

   #110422

   Purpose

   shLuc control, silence Luc gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   Luciferase
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1/TO (RNA PolIII)

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. tet-pLKO.neo_shGFP

   Plasmid

   #110470

   Purpose

   Control, silence GFP gene, doxycycline inducible, neomycin selection

   Depositor

   Sandra Martha Gomes Dias

   Article

   Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

   Insert

   GFP
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1/TO (RNA PolIII)

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. tet-pLKO.puro_shGAC

   Plasmid

   #110423

   Purpose

   shGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1/TO (RNA PolIII)

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. tet operator

   Plasmid

   #8901

   Depositor

   Lynda Chin

   Article

   Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

   Insert

   tet operator
   

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82705

   Purpose

   AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   U6/TO gRNA Expression Cassette Containing Tet2 gRNA
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82703

   Purpose

   AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82701

    Purpose

    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    H1/TO gRNA Expression Cassette Containing Tet2 gRNA
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

    Plasmid

    #117006

    Purpose

    Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA

    Plasmid

    #45789

    Depositor

    Richard Murray

    Inserts

    deGFP
    Tetracycline repressor
    araC
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZS1(pLtetO-TP901-aav-proA-tetR)-katGp-PhiC31-proD-oxyR

    Plasmid

    #78228

    Purpose

    synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

    Insert

    synthetic gene circuit
    

    Available Since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #176273

    Purpose

    Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (sgTet) and BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBtet-GP

    Plasmid

    #60495

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid

    #92099

    Purpose

    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Expression

    Mammalian

    Promoter

    Tet-On 3G Bidirectional

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBtet-BP

    Plasmid

    #60496

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSBtet-RN

    Plasmid

    #60503

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. TETv4

    Plasmid

    #167983

    Purpose

    Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

    Depositor

    Luke Gilbert

    Article

    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

    Insert

    TETv4 (TET1CD-XTEN80-dCas9)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    3xNLS, TET1 catalytic domain, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCasTet-λ

    Plasmid

    #128318

    Purpose

    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

    Depositor

    Christopher Johnston

    Article

    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

    Insert

    TetR and pTetR/TetO
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    Constitutive expression of cas9 and anhydro-tetra…

    Promoter

    tet promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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