We narrowed to 10,295 results for: otos

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ClopHensorN

Plasmid

#50758

Purpose

Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

Depositor

Colin Akerman

Article

Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

Insert

ClopHensorN

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP is EGFP-T203Y

Promoter

CAG

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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PABE-7

Plasmid

#115628

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-nCas9-3*NLS

Use

CRISPR

Expression

Plant

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS5a-OptoCre-REDMAP

Plasmid

#228465

Purpose

Light-inducible Cre recombinase split with REDMAP photoreceptors for bacterial expression

Depositor

Mary Dunlop

Article

Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

Insert

OptoCre-REDMAP

Use

Cre/Lox

Expression

Bacterial

Promoter

pLacUV5

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-CmYLCV

Plasmid

#205245

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site

Use

CRISPR

Expression

Plant

Promoter

CmYLCV

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_CKAR3

Plasmid

#238413

Purpose

AAV construct with CKAR3 expression under a human synapsin promotor and WPRE3 translation enhancer.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

CKAR3 (PKC sensor)

Use

AAV

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-vhhGFP4-hIgG1-Fc

Plasmid

#105576

Purpose

T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)

Use

In vitro transcription

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-tetoff-mVenus-PICdc42

Plasmid

#91876

Purpose

PI(WT): Photo inhibitable (blue light sensitive)

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Tags

mVenus

Expression

Bacterial and Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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HIV-1 integrase F185K/C280S in pET-15b

Plasmid

#61669

Purpose

HIV-1 integrase F185K/C280S in pET-15b

Depositor

Robert Craigie

Article

Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

Insert

HIV-1 integrase F185K/C280S

Use

Synthetic Biology

Tags

His

Promoter

T7 promotor

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pUC57-rm_gCarvi-T2A

Plasmid

#190334

Purpose

Ratiometric reporter for cAMP

Depositor

Naoto Saitoh

Article

Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

ratiometric gCarvi

Use

Unspecified

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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CH-GECO2.1

Plasmid

#52099

Purpose

mCherry-based Calcium indicators

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

CH-GECO2.1

Tags

6 His tag

Expression

Bacterial

Promoter

pBAD promotor

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-10His-StayGold

Plasmid

#211362

Purpose

Mammalian expression of the bright and photostable StayGold fluorescent protein. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

StayGold

Tags

10xHis-tag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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PUMA Frag1-Luc

Plasmid

#16591

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Insert

PUMA promotor fragment (BBC3 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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PL6-CS-WR

Plasmid

#122512

Purpose

construction for sgRNA transcription with Plasmodium falciparum U6 promotor

Depositor

Lubin Jiang

Article

Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

Type

Empty backbone

Use

CRISPR

Promoter

PfU6

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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tagRFP-GPHN.FingR-PhoCl

Plasmid

#236183

Purpose

Half of photoactivatable GFE3. When co-expressed with PBP-E3-HA (Plasmid #236182), ablates inhibitory synapses after exposure to 400 nm light.

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

tagRFP-GPHN.FingR-PhoCl

Expression

Mammalian

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro

Plasmid

#60909

Purpose

A minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

K560-E236A

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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6x Halo-EGFP- 2x PACT

Plasmid

#107265

Purpose

6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

6x Halo-EGFP- 2x PACT

Tags

GFP

Expression

Mammalian

Promoter

T7

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Pink Flamindo-p2a-bPAC

Plasmid

#202716

Purpose

Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Inserts

Pink Flamindo
bPAC

Use

Lentiviral

Tags

10xHis and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNox3

Plasmid

#58341

Purpose

expresses human Nox3 in mammalian cells

Depositor

Botond Banfi

Article

Kiss et al Curr Biol. 2006 Jan 24;16(2):208-13.

Insert

NADPH oxidase 3 (NOX3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pSECRETS-A

Plasmid

#196986

Purpose

For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

Depositor

Eric Josephs

Article

Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pltetO1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PAmKate

Plasmid

#32691

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Expression

Bacterial

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

araB

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPA-mCherry-Kan

Plasmid

#44950

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-mCherry

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pHIV-Wnt1HA

Plasmid

#18983

Depositor

Bryan Welm, Zena Werb

Article

Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

Insert

wingless-related MMTV integration site 1 (Wnt1 Mouse)

Use

Lentiviral

Tags

HA-Tag

Expression

Mammalian

Available Since

Sept. 24, 2008

Availability

Academic Institutions and Nonprofits only
pT7-L1T1L-NLuc

Plasmid

#128942

Purpose

Expresses L1-NLuc gene

Depositor

Takeshi Kobayashi

Article

Kanai et al J Virol. 2019 May 8. pii: JVI.00401-19.

Insert

L1 minor core protein

Tags

Nanoluc

Expression

Bacterial

Promoter

T7 promotor

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CarO-BFP2-TSERex

Plasmid

#124795

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

CarO-BFP2

Expression

Mammalian

Promoter

CAG

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Mac-BFP2-TSERex

Plasmid

#124793

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Mac-BFP2

Expression

Mammalian

Promoter

CAG

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pUC_sgRNA

Plasmid

#68710

Purpose

Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available Since

Sept. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-hNoxa1

Plasmid

#58531

Purpose

expresses human Noxa1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

NADPH oxidase activator 1 (NOXA1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pDropVn

Plasmid

#184873

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pFBNb-cALFA

Plasmid

#159990

Purpose

For the protein expression with ALFA tag at C-terminus in insect cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Insect

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6f-AAV

Plasmid

#182501

Purpose

GFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotor

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Use

AAV

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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