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AY10_pS.Donor.R5.TS

Plasmid

#100292

Purpose

CCR5-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

18 bp RFLP (MluI+KspAI)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-flag-UTR

Plasmid

#183234

Purpose

Mammalian expression empty backbone

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Type

Empty backbone

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK206

Plasmid

#110588

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Nanoluc

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET21a - CPX

Plasmid

#89620

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Cytosolic Tryparedoxin peroxidase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pOPO640

Plasmid

#195296

Purpose

pTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST (Tn7575).

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

miniMcCAST (Tn7575) with KanR

Expression

Bacterial

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN-flag beta-catenin K19R/K49R

Plasmid

#44751

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19R/K49R

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pLdSaCN

Plasmid

#123261

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

gRNA and SaCas9

Use

CRISPR; Leishmania

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a - SodB

Plasmid

#89613

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase B

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-GATA2-DNzf

Plasmid

#1291

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA2 (GATA2 Human)

Expression

Mammalian

Mutation

S263G and M434V

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
SB53

Plasmid

#21488

Depositor

Shoumo Bhattacharya

Article

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Tags

HA

Expression

Mammalian

Mutation

aa1-65 deleted

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
hisSUMO-mZFP57

Plasmid

#87224

Purpose

Express hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

ZFP57 (Zfp57 Mouse)

Tags

hisSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Clover-MyosinIIA-C-18

Plasmid

#56372

Purpose

Localization: Cytoskeleton, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MyosinIIA

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-HDR-sphys-3XHA

Plasmid

#163690

Purpose

plasmid for homologous recombination-mediated insertion of triple HA tag into synaptophysin gene

Depositor

Bernardo Sabatini

Article

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

triple HA tag insertion into endogenous mouse synaptophysin gene

Use

AAV

Tags

triple HA tag

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-GATA2-DC+Nzf

Plasmid

#1293

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA2 (GATA2 Human)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
TA0474_pET21a(+)

Plasmid

#13839

Depositor

SeWon Suh

Article

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

IS200 transposase (Ta0474 Thermoplasma acidophilum)

Expression

Bacterial

Mutation

See Depositor Comments Below

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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721-2µ-staHIS3

Plasmid

#40611

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Budding Yeast)

Tags

HA

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pET11a-DAAT-V33G/T242G

Plasmid

#159278

Purpose

Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

Depositor

Roberto Chica

Article

Parmeggiani et al ACS Catalysis 9, 3482–3486.

Insert

Engineered d-alanine aminotransferase

Tags

6x His tag

Expression

Bacterial

Mutation

Valine 33 and threonine 242 have been mutated to …

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBGT

Plasmid

#62667

Purpose

Donor vector for iRFP knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

iRFP

Use

Cre/Lox

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-TMEg

Plasmid

#61209

Purpose

Fluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-GFP-BCP

Tags

EGFP

Expression

Plant

Promoter

MtBCP1

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-GATA2-DCzf

Plasmid

#1292

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA2 (GATA2 Human)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only