We narrowed to 6,591 results for: nls

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pEGFP-TDP-43 (PY-NLS)

Plasmid

#235490

Purpose

Expression of chimeric human TDP-43-EGFP bearing a PY-nuclear localization sequence (NLS) from HNRNPA1 in place of the native NLS sequence

Depositor

Maxime Rousseaux

Article

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

TARDBP (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

Replaced native NLS with PY-NLS from HNRNPA

Available Since

May 19, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

Plasmid

#52421

Purpose

Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

Depositor

Bertrand Collet

Insert

Fusion NLS-EGFP-P2A-Cherry-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2014

Availability

Academic Institutions and Nonprofits only

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pMpGWB102-Citrine-NLS

Plasmid

#79136

Purpose

Binary vectors for expression of Citrine fluorescent protein with a nuclear localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-NLS

Tags

NLS (Nuclear Localization Signal)

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pEN017-SpCas9-hiNLS-t-M4

Plasmid

#234455

Purpose

Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-LgBiT-FRB

Plasmid

#183403

Purpose

Expresses nuclear-localized LgBiT-FRB

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

LgBiT-FRB

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-NLS-AcrIIA4

Plasmid

#113037

Purpose

Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SV40 NLS-AcrIIA4

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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NLS-SpCas9mSA-His

Plasmid

#182951

Purpose

Express SpCas9mSA in bacteria

Depositor

Wen Xue

Article

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Insert

NLS-SpCas9mSA-His

Use

CRISPR

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pN1-NLS-pHlu

Plasmid

#129320

Purpose

pH measurement, nucleus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

pHluorin (from H. Imamura)

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GFP

Plasmid

#68120

Purpose

Nuclear Localized GFP for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

GFP

Expression

Worm

Promoter

rab-3

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pQC NLS mCherry IX

Plasmid

#37354

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

NLS-mCherry

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Expression

Worm

Promoter

rab-3

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE-NLS-HA-TurboID

Plasmid

#215073

Purpose

Expresses NLS-HA-TurboID in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

TurboID (birA )

Use

Retroviral

Tags

NLS-HA

Expression

Mammalian

Promoter

LTR

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pME-nls-sfGFP

Plasmid

#132973

Purpose

full-length of nuclear localized sfGFP with stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

nuclear localized super folder GFP

Use

Gateway vector

Tags

6x myc and SV40 NLS

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA_Lifeact-GFP_NLS-mCherry

Plasmid

#69058

Purpose

Dual Fluorescent Reporter for F-actin and Nuclei

Depositor

Olivier Pertz

Insert

LifeAct-GFP_IRES_NLS-mCherry

Tags

Lifeact-GFP, NLS-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.C.NWS_mCherry-NLS

Plasmid

#178282

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.C.NWS and Nuclear Localization Signal

Promoter

eF1a

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-2NLS-Cas9

Plasmid

#229773

Purpose

Expresses Cas9 driven by a CMV promoter

Depositor

Yujia Cai

Article

Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

Insert

SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-NLS-mEGFP

Plasmid

#105527

Purpose

T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Nucleoplasmin

Use

In vitro transcription

Tags

mEGFP

Mutation

NLS of nucleoplasmin (encoding amino acids KRPAAT…

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pTetOne-DsRed-cNLS

Plasmid

#138528

Purpose

Inducible expression of DsRed-Express2 with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

DsRed-Express2

Tags

3x SV40 cNLS

Expression

Mammalian

Promoter

TRE3Gs

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-nlsABACUS2-400n

Plasmid

#203726

Purpose

Gateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acid

Depositor

Alexander Jones

Article

Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

Insert

nlsABACUS2-400n

Use

Synthetic Biology

Tags

Myc

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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UAST-uMCas12a+nls2x

Plasmid

#230909

Purpose

inducible expression of Cas12a+ with Gal4.

Depositor

Michael Boutros

Article

Port et al Nat Commun. 2026 Jan 15;17(1):877. doi:

Insert

Cas12a+

Use

CRISPR

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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