We narrowed to 18,492 results for: REV

1. pET-DUET-GST-eIF4G1-His6, eIF4E

   Plasmid

   #37232

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Inserts

   TIF4631 (TIF4631 Budding Yeast)
   CDC33 (CDC33 Budding Yeast)
   

   Tags

   GST and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

   Plasmid

   #196195

   Purpose

   Editing human TSC2 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   TSC2 (TSC2 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pG23A

   Plasmid

   #102884

   Purpose

   Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
   

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Promoter

   Native promoter

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223 GABARAP WT

   Plasmid

   #123080

   Purpose

   Gateway entry clone encoding human GABARAP wild-type

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
   

   Use

   Gateway entry vector / entry clone

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJR025

   Plasmid

   #198832

   Purpose

   Actin filament and cell cortex localization.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::act2-venus::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3540 pMIG Bid p15

   Plasmid

   #8789

   Depositor

   Stanley Korsmeyer

   Article

   Cheng et al Mol Cell 2001 Sep;8(3):705-11.

   Insert

   Bid p15 (Bid Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pSAG1::CAS9-U6::sg290860-6

   Plasmid

   #59855

   Purpose

   Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

   Depositor

   David Sibley

   Article

   Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

   Insert

   CRISPR sg290860-6
   

   Use

   CRISPR; ; toxoplasma gondii

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-6017nes

   Plasmid

   #108655

   Purpose

   A PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   hyBRET-PKA-EV
   

   Tags

   XFP

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV105

   Plasmid

   #63195

   Purpose

   pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-6015nes

    Plasmid

    #108657

    Purpose

    A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-S6K-EV
    

    Tags

    XFP

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSIIbsr-6085x

    Plasmid

    #108653

    Purpose

    A tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    PicchuEV
    

    Use

    Lentiviral

    Tags

    XFP

    Expression

    Mammalian

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPMVAd36

    Plasmid

    #98302

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

    Expression

    Yeast

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pY017 (pcDNA3.1-hPcCpf1)

    Plasmid

    #69989

    Purpose

    Expresses humanized PcCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPcCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAV104

    Plasmid

    #63194

    Purpose

    pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. ALIX(P801G)-Strep

    Plasmid

    #180024

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX(1-868, P801G)-Strep (PDCD6IP Human)
    

    Tags

    Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    P801G

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3538 pMIG Bad

    Plasmid

    #8787

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bad (Bad Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pUpstream2Lox

    Plasmid

    #164573

    Purpose

    Integration of 2 LoxN sites in the genome of P. berghei

    Depositor

    Olivier Silvie

    Inserts

    Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR) (DHFR Human, Synthetic)
    bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU) (FCY1, FUR1 Budding Yeast)
    

    Use

    Cre/Lox

    Promoter

    Bidirectional PbEF1alpha promoter (PBANKA_1133300…

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. prp8-G736E

    Plasmid

    #108450

    Purpose

    prp8-G736E in pRS313 backbone for expression in yeast

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2019 Feb 5;116

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    changed G736 to E

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. UKNP1.4.1

    Plasmid

    #97363

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

    Plasmid

    #191627

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAV123

    Plasmid

    #63189

    Purpose

    pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV Fos YFP MS2

    Plasmid

    #131775

    Purpose

    Fos upstream msfYFP 24xMS2 Fos downstream for CRISPR tagging

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    msfYFP - 24x MS2 loops
    

    Use

    AAV and CRISPR

    Promoter

    n/a

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. UKNP1.2.1

    Plasmid

    #97356

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLenti-hSyn-Gαi*-BERKY3

    Plasmid

    #158428

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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25. pCfB3035(X-4 MarkerFree)

    Plasmid

    #73273

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET28-At_PRORP2-His

    Plasmid

    #67870

    Purpose

    bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP2 (PRORP2 Mustard Weed)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pKH161_FLAG-APEX2-YIPF3 WT

    Plasmid

    #214980

    Purpose

    Flag-APEX2 tagged YIPF3

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    FLAG-APEX2-YIPF3 (YIPF3 Human)
    

    Use

    Lentiviral

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pKH143_EGFP-YIPF3 LIR mut

    Plasmid

    #214975

    Purpose

    GFP tagged LIR mutant YIPF3

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    GFP-YIPF3_mut (YIPF3 Human)
    

    Use

    Lentiviral

    Mutation

    LIR mutant

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pRPL28(K55M) SpHIS5 (pNTI666)

    Plasmid

    #115436

    Purpose

    Expresses K55M RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Expression

    Yeast

    Mutation

    K55M; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET28 sf:mid.11

    Plasmid

    #191921

    Purpose

    Encodes a GFP design, sf:mid.11

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:mid.11
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69M Y145F T167I L220V V224I

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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