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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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T7-VEE-IRES-Puro

Plasmid

#58970

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5B

Plasmid

#61802

Purpose

Human Rab5B fluorescently tagged with GFP on the N-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

Insert

RAB5B (RAB5B Human)

Tags

GFP

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pDIV643

Plasmid

#226182

Purpose

Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

uidA marker flanked with targeting regions to KU70 gene in K.phaffii

Expression

Bacterial

Promoter

TEF1p

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDGM3B

Plasmid

#110809

Purpose

contains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genes

Depositor

David Russell

Article

Khan et al Nat Protoc. 2011 Apr;6(4):482-501. doi:

Insert

AAV3B cap genes, AAV2 rep genes, adenovirus helper genes

Use

AAV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDMS151A_Tet-On-3G

Plasmid

#191577

Purpose

ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

Tet-On(R) 3G

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSGKP_AcsgRNA

Plasmid

#203807

Purpose

pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcgRNA

Use

CRISPR

Promoter

J23119

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pET264-pUC 24xMS2V6 Loxp KANr Loxp

Plasmid

#104393

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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FU-tetO-Gateway

Plasmid

#43914

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

6xHis and V5

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD_IM

Plasmid

#84479

Purpose

Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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TLR4

Plasmid

#20863

Depositor

Jie-Oh Lee

Article

Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

Insert

Toll-Like Receptor 4 (TLR4 Human)

Expression

Insect

Mutation

Contains ectodomain (amino acids 27–631)

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-AAV9cap

Plasmid

#198016

Purpose

For high-level expression of the AAV9 cap gene, suitable for use with pCMV-Rep78/68 SSM library

Depositor

George Church

Article

Jain et al Elife. 2024 Mar 27;12:RP87730. doi: 10.7

Insert

AAV9 cap

Use

AAV

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a_IM

Plasmid

#84478

Purpose

Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBT120b

Plasmid

#122604

Purpose

BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

gIII, luxAB, guide RNA

Use

Synthetic Biology

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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C1-FT-mCitrine-EEVEE-CaaX

Plasmid

#155225

Purpose

MPAct with increased linker flexiblity

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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MD-2

Plasmid

#20864

Depositor

Jie-Oh Lee

Article

Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

Insert

Myeloid differentiation factor 2 (LY96 Human)

Tags

protein A tag

Expression

Insect

Mutation

contains amino acids 19–160

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-GFP-HiBiT

Plasmid

#198317

Purpose

Loads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-GFP-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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CAP-AAV.FUS.3

Plasmid

#222333

Purpose

AAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.

Depositor

Mikhail Shapiro, Jerzy Szablowski

Article

Li et al Nat Commun. 2024 Jun 10;15(1):4924. doi:

Insert

AAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1

Use

AAV

Promoter

p41

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-TMCC2

Plasmid

#121047

Purpose

Expresses human TMCC2 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 2 (TMCC2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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