We narrowed to 22,721 results for: TES

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pcDNA3-HA-DEPTOR-WT

Plasmid

#159159

Purpose

Expresses DEPTOR in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1

Plasmid

#106120

Purpose

Lentiviral expression of ALDH7A1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ALDH7A1 (ALDH7A1 Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSG4K5

Plasmid

#74492

Purpose

For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

Depositor

Xiao Wang

Article

Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pICE-HA-MRE11-H63S

Plasmid

#82035

Purpose

Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

MRE11 (MRE11 Human)

Tags

HA

Expression

Mammalian

Mutation

H63S MRE11 mutant

Promoter

CMV-tet

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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mEGFP-HRas S17N

Plasmid

#18665

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

S17N mutation in H-Ras

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pCMMP-dnPERK-IRES-eGFP

Plasmid

#36954

Depositor

Bill Sugden

Article

Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

Insert

dnPERK (EIF2AK3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

deletion of aa592-1061 to create dominant negative

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-RO1.7-RPE65

Plasmid

#206346

Purpose

AAV plasmid expressing RPE65 in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

Insert

Rpe65 (Rpe65 Mouse)

Use

AAV

Mutation

450-Met (C57BL6 natural variation)

Promoter

1.7kb human red opsin

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pETM33_CRK_SH3

Plasmid

#178472

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CRK_SH3 (CRK Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_fast

Plasmid

#167271

Purpose

Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-GIT1

Plasmid

#15226

Depositor

Rick Horwitz

Article

Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

L425V, E476K and L583P

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pBX_RPL7a_smRuby2_HA

Plasmid

#236087

Purpose

Constitutively encodes RPL7a-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting the 60S subunit.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

RPL7a-smRuby2-HA (RPL7A Synthetic, Human)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(2)-CRY2-mCherry

Plasmid

#160441

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab5a-c-Myc

Plasmid

#79801

Purpose

Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab5a (RAB5A Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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kiCAP-AAV-MDV1A

Plasmid

#196679

Purpose

Rep/Cap plasmid for the production of MDV1A, an AAV capsid with CNS tropism in mice.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RSVGSVY insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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ins:MCS2; cryaa:RFP

Plasmid

#110286

Purpose

A zebrafish insulin promoter containing vector with a second multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Type

Empty backbone

Use

Zebrafish

Promoter

insulin

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-NRAS(G12V)

Plasmid

#35632

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

N-Ras(G12V) (NRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3-V5-PTPN14-wild type

Plasmid

#61003

Purpose

Expresses wild type human PTPN14 in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKbeta1 wt

Plasmid

#31666

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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p026 pCS2-EnR

Plasmid

#11028

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

engrailed (en Fly)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Engrailed repressor domain (bases encoding amino …

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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p8325 pLIX YAP1 WT

Plasmid

#184527

Purpose

Expression of YAP1 WT

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 WT (YAP1 Human)

Use

Lentiviral

Promoter

TRE promoter, Tet ON

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA pCAGGS

Plasmid

#206054

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-FOXP3-IRES-Thy1.1

Plasmid

#17443

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-5U-Venus-PSD-95-3U

Plasmid

#102949

Purpose

Expresses Venus-PSD-95 in mammalian cells

Depositor

Gary Bassell

Article

Ifrim et al J Neurosci. 2015 May 6;35(18):7116-30. d

Insert

PSD-95 (Dlg4 Mouse)

Tags

Venus Fluorescent protein

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3E

Plasmid

#50855

Purpose

expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGL3-ITGA6-1k-Basic-LUC

Plasmid

#107141

Purpose

Luciferase reporter plasmid to monitor transcriptional activity of canine ITGA6-promoter (1kb fragment)

Depositor

Aki Manninen

Article

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Insert

ITGA6-promoter

Use

Luciferase

Expression

Mammalian

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-YOD1

Plasmid

#85664

Purpose

Expression of human YOD1 with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1-S513A/S515A/S516A

Plasmid

#186835

Purpose

Expresses FLAG-BMAL1-S513A/S515A/S516A in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 513/515/516 to Alanine

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAB1679 CMV-HIVNES-GS-Cas13bt3

Plasmid

#176317

Purpose

CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

human codon optimized Cas13bt3

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-BG505.SOSIP-8his

Plasmid

#111847

Purpose

Mammalian expression plasmid for soluble BG505 SOSIP.664

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 Env (BG505 SOSIP.664)

Tags

8his purification tag and CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; has SOSIP mutatio…

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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M77 pIRESpuro-GLUE-hDsh3

Plasmid

#15102

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

Dsh3 (DVL3 Human)

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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