We narrowed to 7,942 results for: RAP
-
Plasmid#166472PurposeHDR template for tagging of endogenous mouse YAP N-terminus with mEGFPDepositorInsertYAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)
ExpressionMammalianAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 ISCU D71A
Plasmid#169880PurposeExpresses ISCU iron-sulfur cluster trapping mutant (dominant negative)DepositorInsertISCU (ISCU Synthetic, Human)
UseLentiviralExpressionMammalianMutationCodon Optimized, D71A iron-sulfur cluster trappin…PromoterTREAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
2XSmBiT-SARS-CoV-2 S1
Plasmid#173430Purposeprotein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 FcDepositorInsert2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)
UseLuciferaseTagshuman IgG1 FcExpressionMammalianMutationfurin cleavage site RRAR to GSASPromoterCMVAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3B Q116P G120
Plasmid#129289PurposeExpresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cellsDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
Tags3xFLAGExpressionMammalianMutationChanged Glutamine 116 to Proline. Deleted amino a…PromoterCMVAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain
Plasmid#187276PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domainDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsmCherryMutationdeltaActin binding domainPromoterU6, CMVAvailable SinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-G6
Plasmid#73437PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.DepositorInsertRepressor G6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107576PurposeB. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic BiologyTagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterpdh promoter Bacillus megaterium DSM319Available SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L-MM
Plasmid#89611PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416DepositorAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLY097-RetroEFS-CD22BBz-PRODH2-WPRE
Plasmid#192198PurposeRetro-CD22CAR-PRODH2DepositorAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-3A2
Plasmid#73435PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.DepositorInsertRepressor 3A2 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain
Plasmid#187275PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domainDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsmCherryMutationdeltaMyosin binding domainPromoterU6, CMVAvailable SinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)
Plasmid#223148PurposeExpression of truncated MECP2 with dCjCas9 and empty gRNA scaffoldDepositorInsertMECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310PromoterEF1aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP
Plasmid#50834PurposeAn AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.DepositorInserthChR2 (H134R)
UseAAV and Cre/LoxTagsEYFPExpressionMammalianMutationH134RPromoterEF1aAvailable SinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1-YAPS127A-deltaCC
Plasmid#166459PurposeExpresses fusion of mEGFP and YAPS127A-deltaCC (delta298-359)DepositorInsertmEGFP, YAPS127A-deltaCC (YAP1 Human)
ExpressionMammalianAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRB_T2098L-VAMP3-mCherry
Plasmid#92429PurposeExpression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine.DepositorInsertVAMP3 (Vamp3 Mouse)
TagsFKBP rapamycin binding (FRB) domain of mTOR with …ExpressionMammalianPromoterCMVAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-sgCD20
Plasmid#209749PurposeLentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CRISPR-V2-gYap-2
Plasmid#166482PurposeExpresses spCas9 and a gRNA targeting mouse YapDepositorInsertgRNA for mouse Yap (Yap1 Mouse)
UseLentiviralAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-4A6
Plasmid#73434PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.DepositorInsertRepressor 4A6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3 2198
Plasmid#49347PurposeThis is actually plasmid pGL3 2185 from the associated publication, which contains a shorter Bmp2 promoter sequence. also contains luciferase reporter.DepositorInsertBmp2 promoter (shorter sequence) (BMP2 Human)
UseLuciferaseTagsluciferasePromoterBmp2 promoterAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP-YAP-N-IDR
Plasmid#166440PurposeBacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion proteinDepositorInsertmEGFP, YAP-N-IDR(1-164) (YAP1 Human)
ExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only