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Addgene

We narrowed to 2,423 results for: LRE

Showing: 1601 - 1650 of 2423 results
  1. pBlastR-MCS-LexA-VP16-MiniWhite

    Plasmid
    #165900
    Purpose
    Blasticidin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning
    Depositor
    Insert
    Selectable Empty Enhancer Driver
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pCDNA3_PKA-Cα(S11D)-mPAGFP

    Plasmid
    #114135
    Purpose
    Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Insert
    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pCAGGS_m-AKAR5

    Plasmid
    #114139
    Purpose
    Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Insert
    AKAR5 C-terminally tagged by KRas C tail
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pCAGGS_cyt-AKAR5

    Plasmid
    #114138
    Purpose
    Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Insert
    AKAR5 with C-terminal nuclear export signal (NES)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pCDNA3_PKA-Cα(S11A)-mPAGFP

    Plasmid
    #114134
    Purpose
    Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Insert
    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pCDNA3_PKA-CαN-mPAGFP

    Plasmid
    #114114
    Purpose
    Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Insert
    the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid
    #170186
    Purpose
    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing
    Depositor
    Insert
    CreERT2-T2A-eBFP2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA3-Lyso-TORCAR

    Plasmid
    #64929
    Purpose
    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.
    Depositor
    Insert
    LAMP1-TORCAR (LAMP1 Human, Synthetic)
    Use
    Tags
    Cerulean, Full-length LAMP1, and YPet
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
  9. pDONR223_HyPer7RgDAAO

    Plasmid
    #217746
    Purpose
    Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.
    Depositor
    Insert
    HyPer7 D amino acid oxidase
    Use
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Promoter
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 1601 - 1650 of 2423 results