GFP-Ago2 S387E mutant lentiviral vector

Ready-to-use AAV Retrograde particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Expresses 6xHis-Ago2 (N-termi tag) in mammalian cells

Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells

Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells

Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells

Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cells

To study effects of acetylation-null tubulin

To study effects of acetylation-null tubulin

Dendra2-based translation reporter

To study effects of acetyl-mimetic (hyper-acetylated) tubulin

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

Dendra2-based translation reporter

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion