325,191 results

1. pCAG_smFP FLAG

   Plasmid

   #59756

   Purpose

   encodes multiple copies of an epitope tag FLAG

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   smFP _FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA4 CoV-2 nsp1 3xFLAG

   Plasmid

   #176057

   Purpose

   Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA4 CoV-2 nsp1 3xFLAG

   Plasmid

   #176057

   Purpose

   Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a T7pCONS TIR-2 sfGFP

   Plasmid

   #154464

   Purpose

   pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a T7pCONS TIR-2 sfGFP

   Plasmid

   #154464

   Purpose

   pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRRLsin-SV40 T antigen-IRES-mCherry

   Plasmid

   #58993

   Purpose

   lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

   Depositor

   Snorri Thorgeirsson

   Article

   Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

   Inserts

   CMV enhancer-chicken beta actin promoter
   SV40 large T antigen
   IRES-mCherry red fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   EcoRV site in MCS converted to BamHI site

   Promoter

   CMV enhancer-chicken beta actin

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRRLsin-SV40 T antigen-IRES-mCherry

   Plasmid

   #58993

   Purpose

   lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

   Depositor

   Snorri Thorgeirsson

   Article

   Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

   Inserts

   CMV enhancer-chicken beta actin promoter
   SV40 large T antigen
   IRES-mCherry red fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   EcoRV site in MCS converted to BamHI site

   Promoter

   CMV enhancer-chicken beta actin

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA 3.2-FUS-1-526aa-V5

   Plasmid

   #29609

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA 3.2-FUS-1-526aa-V5

   Plasmid

   #29609

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pULTRA-CNF

    Plasmid

    #48215

    Purpose

    highly efficient suppressor plasmid

    Depositor

    Peter Schultz

    Article

    Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

    Inserts

    polyspecific MJ tyrosyl synthetase
    tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32L, L65V, F108W, Q109M, D158G, I159A

    Promoter

    proK and tacI

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pULTRA-CNF

    Plasmid

    #48215

    Purpose

    highly efficient suppressor plasmid

    Depositor

    Peter Schultz

    Article

    Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

    Inserts

    polyspecific MJ tyrosyl synthetase
    tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32L, L65V, F108W, Q109M, D158G, I159A

    Promoter

    proK and tacI

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSEVA63_HelperΔgIII

    Plasmid

    #190433

    Purpose

    M13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Insert

    M13 genes I-V and VII-XI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSEVA63_HelperΔgIII

    Plasmid

    #190433

    Purpose

    M13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Insert

    M13 genes I-V and VII-XI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MAC3-tag-N

    Plasmid

    #185482

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/UltralD

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Type

    Empty backbone

    Use

    Tags

    StrepIII-HA-UltraID

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MAC3-tag-N

    Plasmid

    #185482

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/UltralD

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Type

    Empty backbone

    Use

    Tags

    StrepIII-HA-UltraID

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF2631

    Plasmid

    #144140

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    INSM1 (INSM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF2631

    Plasmid

    #144140

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    INSM1 (INSM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pInducer20-EGFP-TEADiv2

    Plasmid

    #228876

    Purpose

    Tet-inducible lentiviral vector for expression of optimized GFP-TEAD inhibitor (TEADiv2)

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

    Insert

    TEADiv2
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pInducer20-EGFP-TEADiv2

    Plasmid

    #228876

    Purpose

    Tet-inducible lentiviral vector for expression of optimized GFP-TEAD inhibitor (TEADiv2)

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

    Insert

    TEADiv2
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSBtet-Pur

    Plasmid

    #60507

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TCE & RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSBtet-Pur

    Plasmid

    #60507

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TCE & RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ArcLight-A242

    Plasmid

    #36857

    Depositor

    Vincent Pieribone

    Article

    Jin et al Neuron. 2012 Sep 6;75(5):779-85.

    Insert

    fluorescent protein voltage sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    semian CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ArcLight-A242

    Plasmid

    #36857

    Depositor

    Vincent Pieribone

    Article

    Jin et al Neuron. 2012 Sep 6;75(5):779-85.

    Insert

    fluorescent protein voltage sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    semian CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAVS1-Bxb1-LP-TC

    Plasmid

    #183754

    Purpose

    AAVS1 targeting construct with the Bxb1 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    loxP-eGFP-attP-Bsd-lox257
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAVS1-Bxb1-LP-TC

    Plasmid

    #183754

    Purpose

    AAVS1 targeting construct with the Bxb1 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    loxP-eGFP-attP-Bsd-lox257
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

    Plasmid

    #193294

    Purpose

    episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SOX2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

    Plasmid

    #193294

    Purpose

    episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Discosoma, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SOX2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GLUT1-eGFP/pcDNA-DEST47

    Plasmid

    #18729

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    pcDNA-DEST47 /hGLUT1-eGFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GLUT1-eGFP/pcDNA-DEST47

    Plasmid

    #18729

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    pcDNA-DEST47 /hGLUT1-eGFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-45b(+)-Tn5

    Plasmid

    #112112

    Purpose

    Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

    Depositor

    Frank Pugh

    Article

    Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

    Insert

    Tn5
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E54K E110K P242A L372P

    Promoter

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge