165,191 results

1. rAAV2/cMG

   Plasmid

   #184539

   Purpose

   AAV packaging plasmid, expressing Rep2 and AAV-cMG capsid

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

   Insert

   AAV-cMG capsid
   

   Use

   AAV; Helper vector for packaging

   Tags

   Expression

   Mutation

   changes on AAV9 capsid: K449R, A589D; insertion o…

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. co luxE pcDNA3.1(+)

   Plasmid

   #129089

   Purpose

   Expression of luxE in mammalian cells

   Depositor

   Stefan Hell

   Article

   Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

   Insert

   co luxE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-FOXD3

   Plasmid

   #153118

   Purpose

   Expression of Flag tagged human FOXD3 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXD3 (FOXD3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSFFV_sfCherry3V(1-10)

   Plasmid

   #117483

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3V(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3V(1-10)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   6 substitutions (K45R, G52D, L83W, T106A, K182R, …

   Promoter

   SFFV

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV GFAP jGCaMP7b

   Plasmid

   #171118

   Purpose

   Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

   Depositor

   Thomas Oertner

   Article

   Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

   Insert

   jGCaMP7b
   

   Use

   AAV

   Tags

   6 His Tag, T7 Tag, and Xpress Tag

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFC332

   Plasmid

   #87845

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   hph (hygromycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV Luciferase PGK-hygro

   Plasmid

   #18782

   Depositor

   Scott Lowe

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. SAFB1-EGFP

   Plasmid

   #32746

   Depositor

   Steffi Oesterreich

   Article

   Townson et al Clin Cancer Res. 2000 Sep;6(9):3788-96.

   Insert

   SAFB1 (SAFB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pRSETb-pcDronpa2

   Plasmid

   #78184

   Purpose

   Expresses pcDronpa2 in E. coli strains

   Depositor

   Peter Dedecker

   Article

   Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

   Insert

   pcDronpa2
   

   Use

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-CMV-puro

    Plasmid

    #131700

    Purpose

    expresses CMV promoter driving puromycin resistance

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al bioRxiv 585984

    Insert

    CMV-puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-NR2B

    Plasmid

    #17925

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    N-methyl D-aspartate receptor 2B (Grin2b Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAKgfplux1

    Plasmid

    #14083

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Bacterial luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK3082

    Plasmid

    #78742

    Purpose

    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    LbCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-nSpCas9-evoCDA1

    Plasmid

    #209042

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    evoCDA1-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPaint-TagBFP-PuroR

    Plasmid

    #80969

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    TagBFP
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX304-Flag-APEX2-NES

    Plasmid

    #92158

    Purpose

    Cytosolic APEX2 construct

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    Flag-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HyPer7.2DAAO-CAV

    Plasmid

    #168303

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the caveolae

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-CAV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CL20_mEGFP_CUX1_NUTM1

    Plasmid

    #205800

    Purpose

    Express mEGFP-tagged fusion protein, CUX1_NUTM1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    CUX1_NUTM1 (CUX1, NUTM1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 3XFlagNICD3

    Plasmid

    #20185

    Depositor

    Raphael Kopan

    Article

    Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

    Insert

    Notch 3 Intracellular Domain (Notch3 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Contains murine Notch 3 Intracellular Domain (Met…

    Promoter

    CMV

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pITGA4-GFP

    Plasmid

    #81178

    Purpose

    Mammalian expression of human full length ITGA4-EGFP

    Depositor

    Chinten James Lim

    Insert

    ITGA4 (ITGA4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains KLGFFKR motif; A1002L (please see deposi…

    Promoter

    CMV

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV V5-hRnf185

    Plasmid

    #175127

    Purpose

    Lentiviral expression of V5-tagged human RNF185

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    RNF185 (RNF185 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. co luxC pcDNA3.1(+)

    Plasmid

    #129087

    Purpose

    Expression of luxC in mammalian cells

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

    Insert

    co luxC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. co luxD pcDNA3.1(+)

    Plasmid

    #129088

    Purpose

    Expression of luxD in mammalian cells

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

    Insert

    co luxD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. co frp pcDNA3.1(+)

    Plasmid

    #129090

    Purpose

    Expression of frp in mammalian cells

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

    Insert

    co frp
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GLP1R-Tango

    Plasmid

    #66295

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GLP1R (GLP1R Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LiRT

    Plasmid

    #214075

    Purpose

    Luminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LiRT_RTctrl

    Plasmid

    #214078

    Purpose

    Luminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LiRTi

    Plasmid

    #214076

    Purpose

    Luminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LiRTX_ctrl

    Plasmid

    #214077

    Purpose

    Luminescent reporter system, NMD negative control. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Remnant of linker sequence present at the end of …

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1(+) EGFP-Rac1(WT)

    Plasmid

    #224278

    Purpose

    Cell transfection and expression of Rac1

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge