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AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171594

Purpose

Strong human ACE2 abundance from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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10279-M28-663

Plasmid

#225662

Purpose

Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Promoter

mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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10279-M28-663

Plasmid

#225662

Purpose

Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Promoter

mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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moxCerulean3

Plasmid

#68066

Purpose

C-terminal fusion to moxCerulean3

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Tags

moxCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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moxCerulean3

Plasmid

#68066

Purpose

C-terminal fusion to moxCerulean3

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Tags

moxCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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AIO-GFP

Plasmid

#74119

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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AIO-GFP

Plasmid

#74119

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CXCL12-sfGFP

Plasmid

#98961

Purpose

Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCL12 (CXCL12 Human)

Tags

Superfolder GFP

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CXCL12-sfGFP

Plasmid

#98961

Purpose

Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCL12 (CXCL12 Human)

Tags

Superfolder GFP

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE GFP

Plasmid

#10668

Purpose

Empty backbone for retroviral gene expression. Select recipient cells using GFP.

Depositor

William Hahn

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pBABE GFP

Plasmid

#10668

Purpose

Empty backbone for retroviral gene expression. Select recipient cells using GFP.

Depositor

William Hahn

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV mCherry-GFP-LC3B WT

Plasmid

#123230

Purpose

Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV mCherry-GFP-LC3B WT

Plasmid

#123230

Purpose

Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEHAC1-HA-BirAonly

Plasmid

#232584

Purpose

Express HA-BirA

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

BirA

Tags

HA

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEHAC1-HA-BirAonly

Plasmid

#232584

Purpose

Express HA-BirA

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

BirA

Tags

HA

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-hlPYK WT

Plasmid

#187975

Purpose

Expresses the human liver pyruvate kinase

Depositor

Audrey Lamb

Article

McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

Insert

hlPYK WT

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-hlPYK WT

Plasmid

#187975

Purpose

Expresses the human liver pyruvate kinase

Depositor

Audrey Lamb

Article

McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

Insert

hlPYK WT

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)

Plasmid

#129405

Purpose

circular Pepper RNA

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

circular F30-Pepper(TAR Variant-2)

Expression

Mammalian

Promoter

U6

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)

Plasmid

#129405

Purpose

circular Pepper RNA

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

circular F30-Pepper(TAR Variant-2)

Expression

Mammalian

Promoter

U6

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf1 wt

Plasmid

#11752

Depositor

Jeff Wrana

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV5B-Flag-Smurf1 wt

Plasmid

#11752

Depositor

Jeff Wrana

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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Flag-HA-USP33

Plasmid

#22601

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP33 (USP33 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP33

Plasmid

#22601

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP33 (USP33 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pGL4 [luc2P/Gli-RE/Hygro] Vector

Plasmid

#236803

Purpose

Express Gli response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

Gli (GLI1 Human)

Use

Luciferase

Mutation

None

Promoter

minP

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGL4 [luc2P/Gli-RE/Hygro] Vector

Plasmid

#236803

Purpose

Express Gli response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

Gli (GLI1 Human)

Use

Luciferase

Mutation

None

Promoter

minP

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET-19b_MMLV-RT

Plasmid

#153312

Purpose

Bacterial expression of MMLV reverse transcriptase

Depositor

Drew Endy, Philippa Marrack

Insert

MMLV Reverse Transcriptase

Tags

10x His

Expression

Bacterial

Available Since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET-19b_MMLV-RT

Plasmid

#153312

Purpose

Bacterial expression of MMLV reverse transcriptase

Depositor

Drew Endy, Philippa Marrack

Insert

MMLV Reverse Transcriptase

Tags

10x His

Expression

Bacterial

Available Since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

Plasmid

#202569

Purpose

Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

LINE-1

Tags

GFP-AI retrotransposition reporter and HaloTag7 o…

Expression

Mammalian

Mutation

Changed ORF1p Arginine 261 to Alanine

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

Plasmid

#202569

Purpose

Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

LINE-1

Tags

GFP-AI retrotransposition reporter and HaloTag7 o…

Expression

Mammalian

Mutation

Changed ORF1p Arginine 261 to Alanine

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF0070

Plasmid

#141505

Purpose

Lentiviral vector for overexpressing the CSDE1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CSDE1 (CSDE1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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