163,765 results

1. hrGFP-Keap1

   Plasmid

   #28025

   Depositor

   Qing Zhong

   Article

   Fan et al Autophagy. 2010 Jul 22. 6(5):.

   Insert

   Keap1 (KEAP1 Human)
   

   Use

   Tags

   hrGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbB8k-csg-NbGFP

   Plasmid

   #166858

   Purpose

   Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.

   Depositor

   Neel Joshi

   Article

   Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469

   Insert

   csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

   Plasmid

   #24334

   Depositor

   Didier Stainier

   Article

   Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

   Insert

   H2B
   

   Use

   Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   mCherry driven by an HSP70 promoterCerulean drive…

   Promoter

   Available since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCN DSB Repair Reporter (DRR)

   Plasmid

   #98895

   Purpose

   DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   Neomycin-T2A-ISce1-ISce1-eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610) (AAV PHP.eB)

   Viral Prep

   #191737-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610) (#191737). In addition to the viral particles, you will also receive purified AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610) plasmid DNA. minBG-driven expression of SYFP2 in striatal direct pathway medium spiny neurons (D1-MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Promoter

   Tags

   SYFP2

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

6. gRNA_Cloning Vector

   Plasmid

   #41824

   Purpose

   An empty gRNA expression vector, used to create a gRNA to a specific sequence.

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   AflII site for Gibson Assembly

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE30-His-T7RNAP

   Plasmid

   #124138

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   T7 RNA polymerase (T7p07 Enterobacteria phage T7)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS31-cCARLIN

   Plasmid

   #160928

   Purpose

   Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)

   Depositor

   Fernando Camargo

   Article

   Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.

   Insert

   CARLIN gRNAs and CAG-GFP-CARLIN array
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for gRNAs; CAG promoter for GFP

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-AP-Ub

   Plasmid

   #232577

   Purpose

   Express Ubiquitin tagged with an Acceptor Peptide (AP)

   Depositor

   Sagar Bhogaraju

   Article

   Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

   Insert

   Ubiquitin
   

   Use

   Tags

   AviTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. PT2/C-Luc//PGK-SB13

    Plasmid

    #20207

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    Flirefly luciferase/Sb transposase
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-mAmetrine-CNA35

    Plasmid

    #61604

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mAmetrine

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mAmetrine and CNA35
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ma-PylRS-lib1

    Pooled Library

    #224585

    Purpose

    Pyrrolysine tRNA synthetase library to generate new genetic code expansion (GCE) systems for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Expression

    Bacterial

    Species

    Methanomethylophilus alvus

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1 phiC31

    Plasmid

    #68310

    Purpose

    In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

    Depositor

    Konrad Basler

    Article

    Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

    Insert

    phiC31
    

    Use

    Rna transcription

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Target-ACEmax

    Plasmid

    #139105

    Purpose

    Mammalian Target-ACEmax expression vector (pSI-625)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-dCas13-NES-eNAT10

    Plasmid

    #238299

    Purpose

    Expresses cytoplasmic version of the programmable RNA acetylation system.

    Depositor

    Won Do Heo

    Article

    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

    Insert

    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    

    Use

    CRISPR

    Tags

    HIV NES (C terminal of dPspCas13b)

    Expression

    Mammalian

    Mutation

    H133A for catalytically inactive mutant, deletion…

    Promoter

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-NLS-dCas13-NLS-eNAT10

    Plasmid

    #238300

    Purpose

    Expresses nuclear version of the programmable RNA acetylation system.

    Depositor

    Won Do Heo

    Article

    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

    Insert

    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    

    Use

    CRISPR

    Tags

    SV40 BPNLS and SV40 BPNLS (C terminal of dPspCas1…

    Expression

    Mammalian

    Mutation

    H133A for catalytically inactive mutant, deletion…

    Promoter

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_P2A_Puro

    Plasmid

    #211364

    Purpose

    Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

    Depositor

    Jacques Neefjes

    Article

    Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6.3-OGDH_wobble

    Plasmid

    #110938

    Purpose

    OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    OGDH wobble cDNA (OGDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    15 silent wobble mutations corresponding to three…

    Promoter

    SV40

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2-Cas9

    Plasmid

    #47322

    Purpose

    For in vivo expression of Cas9 or generation of Cas9 mRNA

    Depositor

    Alex Schier

    Article

    Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

    Insert

    Cas9
    

    Use

    CRISPR; Xenopus and zebrafish expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmGFP-ADAR1-p110

    Plasmid

    #117928

    Purpose

    ADAR1-p110 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p110 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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