167,318 results

1. TFORF3167

   Plasmid

   #144643

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF512B (ZNF512B Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9(1.1)

   Plasmid

   #71814

   Purpose

   Expresses high specificity SpCas9. Px330-like plasmid.

   Depositor

   Feng Zhang

   Article

   Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

   Has Service

   Cloning Grade DNA

   Insert

   enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-jAspSnFR3

   Plasmid

   #203488

   Purpose

   jAspSnFR3 in lentiviral expression vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   jAspSnFR3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Fusion of Zika NS2B-NS3 proteins

   Plasmid

   #204815

   Purpose

   Bacterial expression of a fusion of Zika NS2B-NS3 proteins

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Zika NS2B-NS3 fusion (orf1ab Zika)
   

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. GFP-NPM WT

   Plasmid

   #17578

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   NPM (NPM1 Human)
   

   Tags

   EGFP and Flag

   Expression

   Mammalian

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

   Plasmid

   #107943

   Purpose

   Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
   

   Tags

   3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG PSAM4-5HT3 HC IRES EGFP

   Plasmid

   #119740

   Purpose

   Chemogenetic activator expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Synthetic, Human)
   

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F, R420Q, R424D, R428A

   Promoter

   CAG

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 iCasper T2A HO1

   Plasmid

   #64278

   Purpose

   Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

   Depositor

   Xiaokun Shu

   Article

   To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Insert

   Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Diatom uLoop Assembly Kit

   Plasmid Kit

   #1000000238

   Purpose

   The collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.

   Depositor

   Christopher Dupont

   Available Since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

10. pJR89

    Plasmid

    #140096

    Purpose

    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pADH100

    Plasmid

    #90980

    Purpose

    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

    Plasmid

    #191159

    Purpose

    Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mTagBFP:EGFP.s2:mCherry
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. YES1 (T348I) in pLX303

    Plasmid

    #42564

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YES1 (YES1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    T348I

    Promoter

    CMV

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-Neo-Bam p53 R175H

    Plasmid

    #16436

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R175H (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    CMV

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. human MMP-2 promoter

    Plasmid

    #53432

    Purpose

    promoter activity study

    Depositor

    Etty Benveniste

    Article

    Qin et al J Biol Chem. 1999 Oct 8;274(41):29130-7.

    Insert

    MMP2 promoter (MMP2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    MMP2

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a MDHP_YEAST

    Plasmid

    #204233

    Purpose

    Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHP_YEAST (MDH3 Budding Yeast)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    The last 3 C-terminal residues (SKL) are the pero…

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Mito-V5-BS2 (Mitochondrial matrix)

    Plasmid

    #220344

    Purpose

    Mammalian expression vector BS2 in mitocondria

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Tags

    MTS-matrix and V5 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. V5-BS2-SENP2 (Nuclear Pore)

    Plasmid

    #220345

    Purpose

    Mammalian expression vector BS2 in nuclear pore

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Tags

    SENP2 (nuclear pore localized protein) and V5 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. GAPDH

    Plasmid

    #155622

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    GAPDH (GAPDH Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTNT-pT7-tetR-mCherry

    Plasmid

    #140870

    Purpose

    T7 promoter driven synthesis of TetR-mCherry fusion protein

    Depositor

    Neal Devaraj

    Article

    Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

    Insert

    TetR-mCherry expressed with T7 promoter
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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