We narrowed to 23,628 results for: CRISPR

1. pOC2 Tubb3-GFP KI

   Plasmid

   #183432

   Purpose

   CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and GFP donor
   

   Use

   CRISPR

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113344

   Purpose

   Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   VP64-dCas9-p300core-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLBH547_Tet-Cas14b2Locus

   Plasmid

   #112504

   Purpose

   Expresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b2
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Tet

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ131D

   Plasmid

   #69287

   Purpose

   Golden Gate entry vector; 1st gRNA under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113342

   Purpose

   Expresses dCas9-p300 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   dCas9-p300core-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SGL40C.EFS.E2Crimson

   Plasmid

   #100894

   Purpose

   Lentiviral gRNA delivery

   Depositor

   Dirk Heckl

   Article

   Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

   Insert

   E2Crimson
   

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLH-sgRNA1-boxB-MS2

   Plasmid

   #75396

   Purpose

   sgRNA1-boxB-MS2

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-boxB-MS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

   Plasmid

   #112921

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ155

   Plasmid

   #69306

   Purpose

   Gateway entry vector with AteCas9D10A

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   AteCas9D10A (Arabidopsis codon optimized)
   

   Use

   CRISPR

   Mutation

   D10A mutation; NLS fusion

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCas13 Twin-SUMO

    Plasmid

    #199753

    Purpose

    Expresses codon optimized miniature mCas13 for protein expression in E.coli

    Depositor

    Magdy Mahfouz

    Article

    Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

    Insert

    mCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Promoter

    LacI

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-miniTurbo-Zeo

    Plasmid

    #207736

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. qTAG-N-Zeo-miniTurbo

    Plasmid

    #207703

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miniTurbo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pSB700 lenti gRNA KanR neo

    Plasmid

    #167909

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_9xD3B_Cluc_Norm

    Plasmid

    #68414

    Purpose

    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Cypridina Luciferase-2A-Venus YFP
    

    Use

    Lentiviral and Luciferase

    Mutation

    YFP: M153T,V163A,S175G ("Venus") YFP Va…

    Promoter

    minimalCMV

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330A_D10A-1x4

    Plasmid

    #58774

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCBC-DT1T2.2

    Plasmid

    #134752

    Purpose

    PCR template for sgRNA cloning

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    sgRNA-AtU6_29p
    

    Use

    CRISPR

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCBC-MT1T2.2

    Plasmid

    #134751

    Purpose

    PCR template for sgRNA cloning

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    sgRNA-TaU3p
    

    Use

    CRISPR

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2CT-His-MBP-Ppr_Cas13a_WT

    Plasmid

    #91872

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    PprCas13a (PprC2c2) WT
    

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMOD6-pGRR-r11-r10-yeGFP

    Plasmid

    #74368

    Purpose

    pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    yeGFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    pGRR-r11-r10

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pM115: CasRx control presgRNA

    Plasmid

    #166867

    Purpose

    U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    non-targeting presgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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