We narrowed to 126,239 results for: CIL

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pCS-H2B-cerulean

Plasmid

#53748

Purpose

Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

Cerulean

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-VAMP7

Plasmid

#45913

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 7 (Vamp7 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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mH2A1.1-CT-GFP

Plasmid

#45167

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

Plasmid

#149722

Purpose

Lentiviral expression of crRNA with GFP and a neomycin resistance gene

Depositor

Junwei Shi

Article

Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_MMP-9-HA

Plasmid

#121172

Purpose

Expression of mouse MMP9

Depositor

M. Elizabeth Fini, Shinwu Jeong

Article

Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

Insert

MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)

Tags

HA

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV immediate/early

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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AID-BE3

Plasmid

#100803

Purpose

Expresses AID-BE3 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

activation induced cytidine deaminase (AICDA S. pyogenes, Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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hGli3 flag3x

Plasmid

#84921

Purpose

mammalian expression of Gli3

Depositor

Martin Fernandez-Zapico

Insert

Gli3 (GLI3 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLM494

Plasmid

#100539

Purpose

CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.

Depositor

Bernd Müller-Röber

Article

Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

Inserts

GGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase

Use

Cre/Lox and Synthetic Biology

Expression

Yeast

Promoter

PGK1, TDH3, TIP1, and ZEO1

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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GST-Hsp90 C(626-732)

Plasmid

#22483

Depositor

William Sessa

Article

Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Insert

Hsp90alpha C domain (HSP90AA2P Human)

Tags

GST

Expression

Bacterial

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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3HA-ERHBD-Hoxb8

Plasmid

#212932

Purpose

Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

Depositor

Hans Haecker

Article

Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

Insert

3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Mouse, Human)

Use

Retroviral

Tags

Triple HA tag

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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DNMT3A-dCas9

Plasmid

#100090

Purpose

DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

DNMT3A (DNMT3A Human)

Use

CRISPR

Tags

3XFLag-NLS-DNMT3A-dCas9-NLS

Expression

Mammalian

Mutation

includes aa 602-912

Promoter

CMV

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Luc-pSUPER-Retro-GFP/Neo

Plasmid

#22133

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-hEcto/Tif1g

Plasmid

#20902

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal deletion of 37 aminoacids; Ectodermin …

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
hTRAF2pLPCX-HA-Flag/P874

Plasmid

#20229

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

unmutated NM_021138.3. L replaces M at beginning…

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-L1460P

Plasmid

#69006

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-L1460P (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Leucine 1460 to Proline

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TAK1/F

Plasmid

#44161

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP(S65T)-His3MX6

Plasmid

#41598

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Yeast

Promoter

TEF

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCR-HA-IKKbeta

Plasmid

#15470

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta (Ikbkb Mouse)

Tags

HA

Expression

Mammalian

Available Since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
3341 pcDNA3 Bcl-2 T69A S70A S87A

Plasmid

#13340

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Mutation

changed Threonine 69 to Alanine; changed Serine 7…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

Plasmid

#207664

Purpose

Neuropeptide degradation

Depositor

Sung Han

Article

Kim et al bioRxiv. 2023 Jan 20:2023.01.19.524797.

Insert

NEP (MME Human)

Use

AAV and Cre/Lox

Tags

mRUBY3

Mutation

Addition of signaling peptide (POMC) on the N-ter…

Promoter

hSynapsin

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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