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pWT055e

Plasmid

#96866

Purpose

sgRNA-EMX1

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-EMX1

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-PLtetO1-deGFP-T500

Plasmid

#45392

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Expression

Bacterial

Promoter

PL-tetO1

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pFLIPmal-40uDelta1Venus

Plasmid

#18854

Depositor

Wolf Frommer

Article

Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

Insert

pFLIPmal-40uDelta1V

Tags

CFP and Venus

Expression

Bacterial

Mutation

N227A

Available Since

Oct. 23, 2008

Availability

Academic Institutions and Nonprofits only
pRB36

Plasmid

#72861

Purpose

template base vector for N-terminal tagging, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for introduction of tags

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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E2-Crimson-FKBP(L)

Plasmid

#85001

Purpose

Expresses E2-Crimson-FKBP(L)

Depositor

Benjamin Glick

Article

Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

Insert

E2-Crimson-FKBP(L)

Expression

Yeast

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pFTR5

Plasmid

#167507

Purpose

Broad-Host range expression vector, RSF1010 origin, TetR repressor, PTetA promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTetA

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFTtR5

Plasmid

#167508

Purpose

Broad-Host range expression vector, RSF1010 origin, TtgRAM repressor, PTtg promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TtgRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTtg

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pWT055h

Plasmid

#96867

Purpose

sgRNA-HEK4

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-HEK4

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pKT0174

Plasmid

#8720

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N144I, M153T, V163A

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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Xfz8-pCS2

Plasmid

#32990

Depositor

Sergei Sokol

Article

Itoh et al Mech Dev. 1998 Jun;74(1-2):145-57.

Insert

Xfz8 (fzd8.L Frog)

Expression

Mammalian

Promoter

sCMV IE94

Available Since

Jan. 5, 2012

Availability

Academic Institutions and Nonprofits only
pBS537 tet-hCMV-GFPcre

Plasmid

#11960

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Available Since

Sept. 27, 2006

Availability

Academic Institutions and Nonprofits only
pRB33

Plasmid

#72867

Purpose

template for N-terminal 3x FLAG tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

3xFlag tag

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-fSNAPc

Plasmid

#106986

Purpose

open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

fSNAP

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSK25

Plasmid

#72853

Purpose

template for C-terminal double-FLAG tag, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

double Flag

Expression

Insect

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pFRG5

Plasmid

#149486

Purpose

Broad-Host range expression vector, RSF1010 origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCD175

Plasmid

#113316

Purpose

dCas9, MCP-AsiA, rpoD_F563Y

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, MCP-T4.AsiA, rpoD_F563Y

Use

CRISPR

Expression

Bacterial

Mutation

F563Y

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pIB1

Plasmid

#25450

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pSelect-2

Plasmid

#190242

Purpose

Encodes lacI-targeting gRNA for positive selection of dCas9 activity.

Depositor

Marc Ostermeier

Article

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Expression

Bacterial

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSK15

Plasmid

#72855

Purpose

template for insertion of attP site (C-terminal tag plasmid design), Blasticidin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

attP site

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLitTREPitt

Plasmid

#18661

Depositor

Jakob Reiser

Article

Pluta et al J Gene Med. 2005 Jun . 7(6):803-17.

Insert

TREPitt

Available Since

July 8, 2008

Availability

Academic Institutions and Nonprofits only

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pKT0211

Plasmid

#8734

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECitrine A206R

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with S65G, V68L, Q69M, S72A, T203Y, A206R

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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HS-H4-GFP

Plasmid

#8699

Depositor

Kami Ahmad

Article

Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

Insert

H4 (His4:CG31611 Fly)

Tags

GFP

Expression

Insect

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only

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pAC1822_pCR8-dCasRx

Plasmid

#118656

Purpose

Gateway Donor; dCasRx ORF; No promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx

Use

CRISPR

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBTtG2

Plasmid

#149476

Purpose

Broad-Host range expression vector, pBBR origin, TtgR(AM) repressor, PTtg promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TtgR(AM)-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTtg

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBVG2

Plasmid

#149477

Purpose

Broad-Host range expression vector, pBBR origin, VanR(AM) repressor, PVanCC promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK24

Plasmid

#72852

Purpose

template for C-terminal Twin-Strep tag, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

twin Strep tag

Expression

Insect

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pPEE27

Plasmid

#128367

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the nourseothricin resistance marker (NAT)

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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ZE4 Wnt-8b

Plasmid

#17045

Depositor

Randall Moon

Article

Kelly et al Development. 1995 Jun. 121(6):1787-99.

Insert

wnt-8b (wnt8b Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pKT0101

Plasmid

#8721

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
PCx34.7-pcDNA

Plasmid

#42921

Depositor

John O'Brien

Article

O'Brien et al J Neurosci. 2004 Jun 16;24(24):5632-42.

Insert

Connexin 34.7

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-GluCl_cryst

Plasmid

#31487

Depositor

Eric Gouaux

Article

Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.

Insert

C. elegans glutamate-gated chloride channel alpha, crystallized construct

Use

For rna synthesis for expression in x. laevis ooc…

Tags

8x-His

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pWT055f

Plasmid

#96865

Purpose

sgRNA-FANCF

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-FANCF

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-GluCl_cryst

Plasmid

#31488

Depositor

Eric Gouaux

Article

Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.

Insert

C. elegans glutamate-gated chloride channel alpha, crystallized construct

Tags

8x-His

Expression

Insect

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pSK32

Plasmid

#72854

Purpose

template for C-terminal TEV-site + V5-tag, Puromycin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

TEV-V5 tag

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
6107 pETH6KT218

Plasmid

#1241

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (K218T) (HSP104 Budding Yeast)

Tags

His tag

Expression

Bacterial

Mutation

K218T

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
7093 pNOTAG Hsp104 T317A

Plasmid

#1242

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (T317A) (HSP104 Budding Yeast)

Expression

Bacterial

Mutation

T317A

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pNM3810

Plasmid

#154119

Purpose

mec-4 promoter driving QF in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::QF::act-4 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVG46

Plasmid

#164704

Purpose

yEVenus under the control of PHO5 promoter

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

PHO5pr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pIVR5

Plasmid

#167510

Purpose

Broad-Host range expression vector, pUC origin, Tn7 recognition sites, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLxTd5

Plasmid

#167519

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, tdKatushka2, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-tdKatushka2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKT0212

Plasmid

#8725

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP A206R

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pIM04

Plasmid

#132584

Purpose

pZS2-pLsoxS-TEVp-proD-soxR

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

pLsoxS-TEVp-TermT1
proD-soxR-TermT1

Expression

Bacterial

Promoter

pLsoxS and proD

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SI9-ROBO1

Plasmid

#36144

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

ROBO1 mouse monoclonal antibody (robo1 Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pJF121

Plasmid

#113325

Purpose

pTet-W108 scRNA.b2

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

W108 scRNA_MS2

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS596 tet-hCMV-GFPcre

Plasmid

#11961

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only
pWT016l

Plasmid

#96857

Purpose

(d)theophylline-agRNA-GFP

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)theophylline-agRNA-GFP

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
6011 pETH6K620Tb

Plasmid

#1240

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (K620T mutant) (HSP104 Budding Yeast)

Tags

His tag

Expression

Bacterial

Mutation

K620T

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pIM16

Plasmid

#132583

Purpose

pZA4-oxySp-mCherry-pLsoxS-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

oxySp-mCherry-TermT1
pLsoxS-mCherry-TermT1

Expression

Bacterial

Promoter

oxySp and pLsoxS

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TJF205

Plasmid

#132576

Purpose

pZE1-oxySp-oxyR-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

oxySp-oxyR-mCherry-TermT1

Expression

Bacterial

Promoter

oxySp

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
JRF145

Plasmid

#132579

Purpose

pZE3-pLsoxS-soxR-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

pLsoxS-soxR-mCherry-TermT1

Expression

Bacterial

Promoter

pLsoxS

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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