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HDM_RSV_A_2020_F

Plasmid

#237356

Purpose

Expression plasmid for RSV A F from 2020

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV A F from 2020 human codon optimized from Genbank Accesion PP495954

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCP-tRNA

Plasmid

#133812

Purpose

CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

Depositor

Geraldine Butler

Article

Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

Insert

cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter

Use

CRISPR

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMN-333

Plasmid

#195467

Purpose

Lentiviral Integratable Inducible synZiFTR ZF3 + Reporter

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

inverted[8X ZF3 BS - ybTATA - mCherry] - pSFFV - ZF3 - p65 - ERT2

Use

Lentiviral

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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CRISPR_TRAC

Plasmid

#164993

Purpose

Expression of gRNA targeting TCRalpha constant locus

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against TRAC

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK1

Plasmid

#122672

Purpose

Expresses mouse PCSK1 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

proprotein convertase subtilisin/kexin type 1 (PCSK1) (Pcsk1 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-Bst-LF

Plasmid

#145799

Purpose

Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

Depositor

Andrew Ellington

Article

Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

Insert

Bst-LF

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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CT752

Plasmid

#233658

Purpose

Flp GFP Reporter; FRT-STOP-FRT-GFP

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

FRT-STOP-FRT-GFP

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-gate-3xHA-pGK-PUR

Plasmid

#107393

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

3xHA

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pENO1-iRFP-NATr

Plasmid

#129731

Purpose

Integration plasmid to overexpress iRFP in Candida albicans

Depositor

Robert Wheeler

Article

Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

Insert

iRFP-Candida albicans codon-optimized

Expression

Yeast

Promoter

ENO1 from Candida albicans

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mRuby3

Plasmid

#155221

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Tags

mRuby3

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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DT044A

Plasmid

#159405

Purpose

Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

T7/LacO promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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HA Nprl3 pRK5

Plasmid

#46330

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl3 (NPRL3 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCEP4-AD8gp160

Plasmid

#123260

Purpose

Mammalian expression plasmid for Env from the AD8 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (AD8) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK2

Plasmid

#122673

Purpose

Expresses mouse PCSK2 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) (Pcsk2 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMN-334

Plasmid

#195468

Purpose

Lentiviral Integratable Inducible synZiFTR ZF10 + Reporter

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

inverted[8X ZF10 BS - ybTATA - mCherry] - pSFFV - ZF10 - NS3 - p65

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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NLRP3-mNeonGreen

Plasmid

#200942

Purpose

NLRP3 fused with mNeonGreen to indicate its localization change

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Insert

NLRP3 (NLRP3 Human)

Use

Lentiviral

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-pHlorina

Plasmid

#182763

Purpose

pH-sensitive RFP pHlorina for detection of efferocytosis

Depositor

Robert Campbell

Article

Raymond et al Science. 2022 Mar 11;375(6585):1182-1187

Insert

pHlorina

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC11

Plasmid

#231659

Purpose

Expresses human ZDHHC11 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC11 (ZDHHC11 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCiPS-NIFV

Plasmid

#110807

Purpose

Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells

Depositor

David Russell

Article

Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

Insert

Pol

Expression

Mammalian

Mutation

2 mutations in the DDE catalyic core motif of the…

Promoter

CMV

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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