We narrowed to 26,922 results for: STO;

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pSG5-Myr-FLAG-p110β-DM

Plasmid

#55719

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 beta

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

S205D, K224A

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pCfB3043(gRNA XI-1)

Plasmid

#73285

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pACYC-OspCas12c-noncoding

Plasmid

#120878

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-OspCas12

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHL 1277

Plasmid

#53017

Purpose

AmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Inserts

GFP
mCherry

Use

Synthetic Biology

Tags

RBS(st7)

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pCfB3049(gRNA XII-4)

Plasmid

#73291

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_h7SK

Plasmid

#102846

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert SAM_h7SK

Use

Pcr template for stagr inserts

Promoter

H7SK

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pUHD 10.3-human MIZ1 delta POZ

Plasmid

#74160

Purpose

Tet inducible expression of MIZ1 delta POZ

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MIZ1 delta POZ (ZBTB17 Human)

Expression

Mammalian

Mutation

delta POZ domain (first 109 aa)

Promoter

CMV2

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CSNK2A1

Plasmid

#67085

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CSNK2A1 (CSNK2A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
JM3 [JBEI-6891]

Plasmid

#89788

Purpose

Broad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control of BAD promoter

Depositor

Harry Beller

Article

Muller et al Appl Environ Microbiol. 2013 Jul;79(14):

Insert

tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)

Expression

Bacterial

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-SAR1-V5-His6

Plasmid

#67430

Purpose

Expression of hSar1 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-C1X-Nup88-mut

Plasmid

#87337

Purpose

To express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pKS-DTA miR-106b~25

Plasmid

#19030

Depositor

Tyler Jacks

Article

Ventura et al Cell. 2008 Mar 7. 132(5):875-86.

Insert

microRNA 106a-25 (Mir25 Mouse)

Use

Mouse Targeting

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoGC

Plasmid

#100031

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC

Tags

1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)

Expression

Mammalian

Promoter

Adenovirus major late promoter

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF1-PTPRD-G1707R

Plasmid

#32526

Depositor

Todd Waldman

Article

Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

Insert

Protein tyrosine phosphatase receptor-type delta G1707R (PTPRD Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G1707R

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pU6Rosa-CAG-Gal4-Rad18UBD

Plasmid

#119012

Purpose

Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Gal4 (GAL4 Budding Yeast)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF1-PTPRD-P1690F

Plasmid

#32525

Depositor

Todd Waldman

Article

Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

Insert

Protein tyrosine phosphatase receptor-type delta P1690F (PTPRD Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P1690F

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CTNNB1

Plasmid

#67119

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CTNNB1 (CTNNB1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
BB1_23_eGFP

Plasmid

#98518

Purpose

BB1 with insert: CDS eGFP

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

eGFP

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHL 1278

Plasmid

#53018

Purpose

AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Inserts

mCherry
GFP

Use

Synthetic Biology

Tags

RBS(st7)

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-SZT2 2241-end

Plasmid

#87039

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Tags

Flag

Expression

Mammalian

Mutation

AA 2241-end

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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