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lentiCRISPR v2-sgCrls1-2#

Plasmid

#234766

Purpose

knockout Crls

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Crls1 (Crls1 Mouse)

Use

Lentiviral

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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RALA V25L

Plasmid

#122927

Purpose

Bacterial expression of RALA V25L

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

V25L

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA V25M

Plasmid

#122928

Purpose

Bacterial expression of RALA V25M

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

V25M

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA D130G

Plasmid

#122929

Purpose

Bacterial expression of RALA D130G

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

D130G

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA S157A

Plasmid

#122930

Purpose

Bacterial expression of RALA S157A

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

S157A

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA R176X

Plasmid

#122931

Purpose

Bacterial expression of RALA R176X

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

R176X

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA WT

Plasmid

#122925

Purpose

Bacterial expression of RALA WT

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA G23D

Plasmid

#122926

Purpose

Bacterial expression of RALA G23D

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

G23D

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBOB-EF1-FastFUCCI-Puro

Plasmid

#86849

Purpose

3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

Depositor

Kevin Brindle, Duncan Jodrell

Article

Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

Inserts

mKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1 and PGK

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_eCB2.0

Plasmid

#164604

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

hSyn

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EGFP-IRES-Neo

Plasmid

#128660

Purpose

mammalian expression of EGFP with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

EGFP

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC5-Tmem192-3xHA

Plasmid

#102930

Purpose

Lentiviral expression of a lysosomal tag (Tmem192-HA)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

Tmem192 (TMEM192 Human)

Use

Lentiviral

Tags

HA

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA

Plasmid

#156436

Purpose

For transient expression of human PDS5B tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human PDS5B (PDS5B Human)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2-TVA-eGFP-N2cG

Plasmid

#175439

Purpose

Cre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1142 (pSPIN, pBBR1 backbone)

Plasmid

#160730

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pHSE401

Plasmid

#62201

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

gRNA scaffold
zCas9

Use

Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

maize codon optimized

Promoter

35S and AtU6-26p

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

Plasmid

#154473

Purpose

Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

SFFV

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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TRE-VEGFA EF1a-rtTA3

Plasmid

#195881

Purpose

Lentiviral construct of doxycycline inducible VEGFA

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

VEGFA (Vegfa Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3GS

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLJC5-Tmem192-2xFlag

Plasmid

#102929

Purpose

Lentiviral expression of a lysosomal tag (Tmem192-Flag)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

Tmem192 (TMEM192 Human)

Use

Lentiviral

Tags

Flag

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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Lifeact-mScarlet3-S2

Plasmid

#246873

Purpose

Expression of mScarelt3-S2 fused with Lifeact. This construct is used for imaging actin filaments.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Tags

Lifeact

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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