We narrowed to 29,721 results for: REP

1. PLJR965

   Plasmid

   #115163

   Purpose

   Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

   Depositor

   Sarah Fortune

   Article

   Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

   Inserts

   Sth1 sgRNA scaffold
   Sth1 dCas9
   TetR (deleted TetON) MtbCO
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP6-VEE-GFP

   Plasmid

   #58976

   Purpose

   expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   EGFP
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   26S subgenomic promoter

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. 8522-M01-663

   Plasmid

   #225660

   Purpose

   Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

   Depositor

   Dominic Esposito

   Article

   Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

   Insert

   dsCopGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SORE6-mCMVp

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMX-eSOX17FNV

   Plasmid

   #118838

   Purpose

   Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

   Depositor

   Ralf Jauch

   Article

   Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

   Insert

   eSOX17FNV (Sox17 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   FNV: L46F, V53N, E57V

   Promoter

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPRx-Sth1Cas9-L5

   Plasmid

   #140993

   Purpose

   Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

   Depositor

   Wilbert Bitter

   Article

   Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

   Inserts

   Sth1Cas9
   TetR
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified; Integrative in mycobactreia

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p11d-tRPA(123)

   Plasmid

   #102613

   Purpose

   Expression of human RPA in E. coli

   Depositor

   Marc Wold

   Article

   Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

   Inserts

   RPA1 (RPA1 Human)
   RPA2 (RPA2 Human)
   RPA3 (RPA3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR-cBEST

   Plasmid

   #125689

   Purpose

   C to T base editor for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

   Insert

   streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   ermE*/tipA

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cas9-sgRNA-A+B

   Plasmid

   #149370

   Purpose

   Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

   Depositor

   Tarek Abbas

   Article

   Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

   Inserts

   Cas9
   sgRNA-A
   sgRNA-B
   Puromycin resistance
   

   Use

   CRISPR

   Tags

   3xFLAG, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh, PGK, and U6

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. iNos-eGFP

   Plasmid

   #207251

   Purpose

   Reporter for expression of eGFP under control of iNos promoter

   Depositor

   Michelle Farkas

   Article

   Mas-Rosario et al Front Oncol. 2023 Apr 5;13:1151384. doi:

   Insert

   iNos Promoter (Nos2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPEY-GAL-Nat-ADE2

    Plasmid

    #232103

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. 8522-M31-663

    Plasmid

    #225661

    Purpose

    Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SORE6-mCMVp

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ223 (dAsCpf1-SRDX)

    Plasmid

    #86209

    Purpose

    Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    dAsCpf1
    

    Use

    Gateway compatible cpf1 vectors

    Tags

    NLS and SRDX repressor and NLS

    Expression

    Plant

    Mutation

    D908A

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cas9-sgRNA-A

    Plasmid

    #149369

    Purpose

    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    Cas9
    sgRNA-A
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    3xFLAG, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, PGK, and U6

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDblet

    Plasmid

    #8848

    Depositor

    Joel Huberman

    Article

    Brun et al Gene. 1995 Oct 16. 164(1):173-7.

    Insert

    ARS doublet
    

    Use

    Yeast cloning vector

    Tags

    Expression

    Yeast

    Mutation

    This vector contains, between AatII sites, a dire…

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMX-eSOX17WHC

    Plasmid

    #118839

    Purpose

    Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSOX17WHC (Sox17 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    WHC: L46W, V53H, E57C

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGM55

    Plasmid

    #71249

    Purpose

    luciferase reporter with hGM-CSF -55 to +28 minimal promoter

    Depositor

    Peter Cockerill

    Article

    Bert et al Plasmid. 2000 Sep;44(2):173-82.

    Insert

    GM-CSF minimal promoter (-55 to +28) (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GM-CSF minimal

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS306Sup35R1

    Plasmid

    #17715

    Depositor

    Susan Lindquist

    Article

    Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

    Insert

    pRS306Sup35R1 (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    An pRS306 based integrating plasmid designed for …

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPEY-GAL-Hyg-ADE2

    Plasmid

    #232104

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPEY-GAL-Kan-ADE2

    Plasmid

    #232105

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEM5Z(+)-EGFP

    Plasmid

    #65206

    Purpose

    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.

    Depositor

    LuZhe Sun

    Article

    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Restriction enzyme recognition sites added either…

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only