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We narrowed to 13,558 results for: cas9 genes

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SpyCas9-AIDx

Plasmid

#135343

Purpose

Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KanR neo

Plasmid

#167869

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Tags

Expression

Mutation

D10A, D839A, H840A, N863A

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3TOPO-T7-hCas9

Plasmid

#161876

Purpose

In vitro transcription of SpCas9

Depositor

Stephane Pelletier

Article

Pelletier et al Immunity. 2015 Jan 20;42(1):18-27. doi:

Insert

human codon optimized SpCas9

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCas9-J23109

Plasmid

#113150

Purpose

Plasmid constitutively expressing Cas9 protein

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1a-nSpCas9-NG

Plasmid

#171692

Purpose

Expresses nSpCas9-NG in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

nSpCas9-NG

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

Promoter

EF1a

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-nSpCas9

Plasmid

#171691

Purpose

Expresses nSpCas9 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

nSpCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A

Promoter

EF1a

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_KanR neo

Plasmid

#167861

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pdCas9-PBE

Plasmid

#98161

Purpose

Expresses CRISPR base editor in protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-dCas9-UGI-NLS

Use

Tags

Expression

Plant

Mutation

Promoter

Ubi

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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AV44_pCAG.Cas9-D10A.gRNA.S1

Plasmid

#199258

Purpose

Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9-D10A nickase
AAVS1-targeting gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter and RNA polymerase III promoter for …

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pdCas9-M-3A2

Plasmid

#73435

Purpose

CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Repressor 3A2 (orthogonal T7-lac repressor)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Constitutive wild-type S. pyogenes promoter

Available since

March 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-SauCas9

Plasmid

#174040

Purpose

In vitro transcription of SauCas9 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

SauCas9

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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AB65_pCAG.Cas9-D10A.rBGpA

Plasmid

#199256

Purpose

Expression construct encoding the SpCas9-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

SpCas9.D10A nickase

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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p3s-Cas9-HN

Plasmid

#104171

Purpose

Cas9-WT mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

Cas9-WT

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_EE-nCas9

Plasmid

#163541

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

EE-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)

Promoter

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pFGC-I2Cas9

Plasmid

#173158

Purpose

Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.

Depositor

Piotr Ziolkowski

Article

Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

Inserts

hSpCas9
pNAP:dsRED:tNOS
pMAS:BAR:tMAS
ICU2 upstream regulatory region

Use

Tags

3xFLAG-NLS and NLS

Expression

Plant

Mutation

Contains a D169N mutation with no visible effect …

Promoter

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pdCas9 (GB1079)

Plasmid

#75399

Purpose

Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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ABEMax nSaCas9

Plasmid

#135364

Purpose

Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax nSaCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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AV15_pCAG.Cas9.gRNA.S1

Plasmid

#199259

Purpose

Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9 nuclease
AAVS1-targeting gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG promoter and RNA polymerase III promoter for …

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-HA-dSpCas9

Plasmid

#92112

Purpose

Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9

Use

CRISPR

Tags

HA tag and NLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Cbh

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pdCas9-AsiA

Plasmid

#158063

Purpose

Expressing CasTA of dCas9 fused AsiA

Depositor

Harris Wang

Article

Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

Insert

dCas9-AsiA

Use

Tags

Expression

Bacterial

Mutation

Promoter

TetO

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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