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pcDNA3-Tsapphire-StagRFP-SrcAR

Plasmid

#173018

Purpose

Long-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

Tsapphire-StagRFP-SrcAR

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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SaCas9 TadDE

Plasmid

#193846

Purpose

Express TadDE (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE with SaCas9

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A1

Plasmid

#132034

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A1 (SLC22A1 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-i3 G183S ONE-GO

Plasmid

#204338

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3 G183S ONE-GO

Use

Lentiviral

Mutation

G183S mutation in Galpha-i3 (renders it RGS insen…

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-PH1(WT)

Plasmid

#86599

Purpose

Expresses eGFP-tagged PH(WT) domain of human Akt1

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 PH domain (AKT1 Human)

Expression

Mammalian

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-His-MinD_MinE

Plasmid

#133621

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

Depositor

Petra Schwille

Article

Loose et al Science. 2008 May 9;320(5877):789-92. do

Insert

His-MinD_MinE (minD, minE E. coli)

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV4a-SIRT6-H133Y-FLAG

Plasmid

#102324

Purpose

Human SIRT6 H133Y with FLAG tag for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed histidine 133 to tyrosine

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Mito-CaNAR2

Plasmid

#64731

Purpose

Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2

Tags

Cerulean3, N-terminal 30 amino acids from DAKAP1,…

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKII

Plasmid

#45064

Purpose

Expression of CaMKII

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

CaM Kinase II (Camk2a Rat)

Expression

Mammalian

Promoter

RSV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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myc-hIFITM3-delta59-68

Plasmid

#104361

Purpose

Expresses human IFITM3-delta59-68 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

Insert

IFITM3 (IFITM3 Human)

Tags

myc

Expression

Mammalian

Mutation

deleted amino acids 59 through 68

Promoter

CMV

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Hoxc6

Plasmid

#45553

Depositor

Jeremy Dasen

Article

Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

Insert

Hoxc6 (Hoxc6 Mouse)

Promoter

CMV / b-actin

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only
pLKO.1-sh-mSOX9-2

Plasmid

#40645

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSOX9-2 (Sox9 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
mNeongreen-aGBD(anillin)

Plasmid

#129633

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RhoA binding domain of anillin (712-786)

Expression

Mammalian

Promoter

CMVdel

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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DAXX-myc-his

Plasmid

#1852

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

DAXX (Daxx Mouse)

Tags

his and myc

Expression

Mammalian

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pOZ-N-FH Abraxas

Plasmid

#27495

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

Abraxas (FAM175A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-DAKAP-μDpTT

Plasmid

#182324

Purpose

Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

DAKAP-μDronpa-TagRFP-T

Tags

N-terminal 30 amino acids from DAKAP1

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pVETL-Cmcs-Flag-Ataxin3Q80

Plasmid

#22120

Depositor

Henry Paulson

Article

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pILGFPE9

Plasmid

#83545

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter-yEGFP

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRS1422

Plasmid

#12548

Depositor

Robert Sobol

Article

Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

Insert

human Methylpurine DNA glycosylase (MPG Human)

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-FL

Plasmid

#120889

Purpose

Expresses wildtype Ptch1 for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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