We narrowed to 18,503 results for: REV

1. pcDNA3-Cyto-ExRai-AktAR2

   Plasmid

   #184048

   Purpose

   Cytosol-targeted ExRai-AktAR2.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Cyto-ExRai-AktAR2
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti SYN-dCas9-KRAB-MeCP2

   Plasmid

   #155365

   Purpose

   Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

   Depositor

   Jeremy Day

   Article

   Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   3x FLAG and 7x His

   Expression

   Mammalian

   Promoter

   SYN

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINDUCER21-puro_TAOK3

   Plasmid

   #209778

   Purpose

   doxycycline-inducible expression of TAOK3

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   TAOK3 (TAOK3 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103_YES1-1/1

   Plasmid

   #91439

   Purpose

   Protein expression and purification of human SH3 domain construct YES1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   YES1-1/1 (YES1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-H2B-AID-EYFP

   Plasmid

   #47329

   Purpose

   for plasmid integration using the Flp-In system. AID at C-terminal

   Depositor

   Don Cleveland

   Article

   Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

   Insert

   histone H2B (H2BC21 Human)
   

   Tags

   AID and EYFP

   Expression

   Mammalian

   Available Since

   Aug. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA 3.1 AnxA6

   Plasmid

   #20407

   Depositor

   Carlos Enrich

   Article

   Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

   Insert

   AnxA6 (Anxa6 Rat)
   

   Expression

   Mammalian

   Available Since

   May 1, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pCMV 3xFLAG-LC3A WT

   Plasmid

   #123089

   Purpose

   Expresses 3xFLAG-LC3A wild-type in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p35S:Citrine-TurboID-3xHA

   Plasmid

   #199243

   Purpose

   This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

   Insert

   Citrine-TurboID
   

   Tags

   3xHA and TurboID-3xHA

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTP-PCR C3

   Plasmid

   #189620

   Purpose

   Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

   Insert

   Reduced Mitochondrial Genome of Thalassiosira pseudonana
   

   Use

   Synthetic Biology; Diatom expression

   Expression

   Bacterial and Yeast

   Mutation

   Removed repeat region, 10-kbp deletion, and minor…

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUBQ:Citrine-TurboID-3xMyc

    Plasmid

    #199244

    Purpose

    This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

    Insert

    Citrine-TurboID-3xMyc
    

    Tags

    TurboID-3xMyc

    Expression

    Plant

    Promoter

    Arabidopsis UBQ10 promoter with intron

    Available Since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SNAP-OMP25

    Plasmid

    #141149

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Budding Yeast, Rat)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Akt-STOPS

    Plasmid

    #175006

    Purpose

    Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMC234r-ccdB/TPK2

    Plasmid

    #176179

    Purpose

    yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    dropout cassette containing toxic genes ccdB and TPK2
    

    Use

    Synthetic Biology

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-Puro-sgNT

    Plasmid

    #138681

    Purpose

    Expresses a non-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNT
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcGFP1-N1-SialT

    Plasmid

    #87324

    Purpose

    To express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferase

    Depositor

    Lei Lu

    Article

    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

    Insert

    ST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV 3xFLAG-GABARAPL2 WT

    Plasmid

    #123103

    Purpose

    Expresses 3xFLAG-GABARAPL2 wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBa. BDNF-Halo

    Plasmid

    #162718

    Purpose

    Mammalian expression of rat BDNF

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Brain derived neurotrophic factor (Bdnf Rat)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF5B-FRT-AP-Sstr3-GFP-DEST

    Plasmid

    #49098

    Purpose

    Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    Sstr3 (Sstr3 Mouse)
    

    Tags

    GFP and acceptor peptide (AP)

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2,0_3M5M_eGFP

    Plasmid

    #135474

    Purpose

    Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    Marburg virus minigenome, eGFP reporter
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    T7

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ntn1-Fc-His

    Plasmid

    #72104

    Purpose

    Expresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn1 (Ntn1 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAT15516_BEAR-GFP-2in1

    Plasmid

    #174082

    Purpose

    BEAR-GFP plasmid with a gRNA that targets its own plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. lenti CAG-FLAG-dCas9-VPR

    Plasmid

    #114197

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the CAG promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCDNA3_Sec61B-V5-APEX2

    Plasmid

    #83411

    Purpose

    Expressed C-term tagged APEX2 on Sec61B in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    Sec61B-V5-APEX2 (SEC61B Synthetic, Human)
    

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Promoter

    CMV-F

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pOPINK-OTUD2 (full-length, aa 1-348)

    Plasmid

    #61409

    Purpose

    Expresses human OTUD2 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD2 (YOD1 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. pENTR-MBD1

    Plasmid

    #47057

    Purpose

    Expresses methyl-CpG binding domain protein 1

    Depositor

    Huda Zoghbi

    Article

    Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

    Insert

    Methyl-CpG Binding Domain Protein 1 (MBD1 Human)
    

    Use

    Gateway entry vector

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pLV V5-hRnf185

    Plasmid

    #175127

    Purpose

    Lentiviral expression of V5-tagged human RNF185

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    RNF185 (RNF185 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP

    Plasmid

    #174876

    Purpose

    PiggyBac transposon vector for expression of RNAPII Ser2ph mintbody

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    RNAPII Ser2ph mintbody-SNAP
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

    Plasmid

    #86421

    Purpose

    N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    CDC42pr-KAR2SS-GFP1-10
    

    Tags

    GFP1-10

    Expression

    Yeast

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. CALCOCO1-HA

    Plasmid

    #137766

    Purpose

    Expression and localization of tagged CALCOCO1

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    CALCOCO1 (CALCOCO1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pUC57-Amp_CRISPIE donor B5

    Plasmid

    #172846

    Purpose

    CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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