We narrowed to 150,751 results for: ins

1. ST-Tstem-GFP

   Plasmid

   #162501

   Purpose

   Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   ST6Gal1 mutant (Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The Tac stem region is inserted within ST6Gal1

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. vPyACR_2164382

   Plasmid

   #153029

   Purpose

   Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

   Insert

   vPyACR_2164382
   

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   vPyACR_2164382 was C-terminally truncated to incl…

   Promoter

   CMV (+ enhancer)

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. vPyACR_21821

   Plasmid

   #153030

   Purpose

   Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

   Insert

   vPyACR_21821
   

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   vPyACR_21821 was C-terminally truncated to includ…

   Promoter

   CMV (+ enhancer)

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN_TT 3xFLAG-MAVS-Ala148

   Plasmid

   #158630

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Mutation

   Q148A

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM33_Nsp3c_SUD-C

   Plasmid

   #156471

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3c_SUD-C (ORF1ab Synthetic)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST40-2XHA-Src-S4-UD-mCherr

   Plasmid

   #140307

   Purpose

   Expresses Flag-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   contains only the SH4 and UD regions fused to mCh…

   Promoter

   CMV

   Available Since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-AAV2-hSyn-MerMAID1-Citrine

   Plasmid

   #126520

   Purpose

   Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

   Insert

   MerMAID1
   

   Use

   AAV

   Tags

   Citrine

   Promoter

   human synapsin

   Available Since

   Nov. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p-mCherry-C1-MerMAID2

   Plasmid

   #126514

   Purpose

   Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

   Insert

   MerMAID2
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV (+ enhancer)

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-mCherry-C1-MerMAID5

   Plasmid

   #126517

   Purpose

   Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

   Insert

   MerMAID5
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV (+ enhancer)

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-mCherry-C1-MerMAID3

    Plasmid

    #126515

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID3
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+ enhancer)

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p-mCherry-C1-MerMAID6

    Plasmid

    #126518

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID6
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+ enhancer)

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p-mCherry-C1-MerMAID4

    Plasmid

    #126516

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID4
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+ enhancer)

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-mCherry-C1-MerMAID7

    Plasmid

    #126519

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID7
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+ enhancer)

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-mCherry-C1-MerMAID1

    Plasmid

    #126513

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID1
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+ enhancer)

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. eGFP-PRM-3R

    Plasmid

    #112156

    Purpose

    Mammalian eGFP expression plasmid for PRM-3R.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    PRM-3R (ABL1 Human)
    

    Expression

    Mammalian

    Mutation

    contains only the PRM domain of ABl1

    Promoter

    CMV

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIDS-LSD1-C

    Plasmid

    #109160

    Purpose

    Encodes a human C-terminal LSD1 fragment to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human C-terminal LSD1, sequence-optimized for insect cells (KDM1A Human)
    

    Expression

    Insect

    Promoter

    p10

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AF10_OM-LZ

    Plasmid

    #111753

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    MLLT10 (MLLT10 Human)
    

    Tags

    6x His - TEV

    Expression

    Bacterial

    Mutation

    Contains the OM-LZ region of AF10 (residues 720-7…

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pKK-TEV-mCardinal

    Plasmid

    #105798

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCardinal

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKK-TEV-mCerulean

    Plasmid

    #105790

    Purpose

    Expression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCerulean

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKK-mCardinal-TEV

    Plasmid

    #105781

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mCardinal-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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