We narrowed to 18,503 results for: REV

1. pDONR223 LC3A WT

   Plasmid

   #123069

   Purpose

   Gateway entry clone encoding human MAP1LC3A wild-type

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Use

   Gateway entry vector / entry clone

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. anti-FIX(a) Emi HC

   Plasmid

   #113664

   Purpose

   For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FX(a) Emi HC and the Emi LC.

   Depositor

   Robert Peters

   Article

   Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1

   Insert

   Emicizumab anti-FIX/FIXa heavy chain
   

   Expression

   Mammalian

   Promoter

   CMV Promoter

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174882

   Purpose

   Targeting vector for generating Nanog STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Nanog
   

   Use

   Mouse Targeting

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCherry-NES-SspB-MCS-P2A-iLIDcaax

   Plasmid

   #173869

   Purpose

   Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-MCS-P2A-iLIDcaax
   

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHH0103_TXK-1/1

   Plasmid

   #91539

   Purpose

   Protein expression and purification of human SH3 domain construct TXK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   TXK-1/1 (TXK Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EZH2 E10.3 gRNA

   Plasmid

   #90685

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EZH2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   EZH2 (Guide Designation E10.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pScRad52-SpCas9 (pXK-1646)

   Plasmid

   #208823

   Purpose

   CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.

   Depositor

   Kun Xu

   Article

   Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

   Type

   Empty backbone

   Use

   CRISPR; Cas9

   Tags

   Rad52

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Sema6c-AP-His

   Plasmid

   #72041

   Purpose

   Expresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema6c (Sema6c Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCfB2909(XII-5 MarkerFree)

   Plasmid

   #73281

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Golgi-ExRai-AktAR2

    Plasmid

    #184051

    Purpose

    Golgi-targeted ExRai-AktAR2.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Golgi-ExRai-AktAR2
    

    Tags

    N-terminal 35 amino acids from human endothelial …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pECE M2-SNX17 WT

    Plasmid

    #69811

    Purpose

    Expresses M2-SNX17 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Sorting nexin-17 (SNX17 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Sema6d.4-AP-His

    Plasmid

    #72044

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.4 (Sema6d Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOPINK-OTUD6A (full-length, aa 1-288)

    Plasmid

    #61416

    Purpose

    Expresses human OTUD6A (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6A (OTUD6A Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. POLArISact

    Plasmid

    #164971

    Purpose

    Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA3_V5-APEX2-Sec61B

    Plasmid

    #83412

    Purpose

    Expressed N-term tagged APEX2 on Sec61B in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    V5-APEX2-Sec61B (SEC61B Human, Synthetic)
    

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Promoter

    CMV-F

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-Syn-AlstR-IRES2-EGFP

    Plasmid

    #14895

    Depositor

    Edward Callaway

    Article

    Tan et al Neuron. 2006 Jul 20. 51(2):157-70.

    Insert

    AlstR (AstA-R1 Fly)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 11, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCPP5233

    Plasmid

    #128715

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

    Insert

    shcM-hopM1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-Akt-STOPS-NES

    Plasmid

    #175008

    Purpose

    Cytosol-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pProEx Ypt1(S17C) CL

    Plasmid

    #224265

    Purpose

    Protein purification of Ypt1 (S17C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Ypt1 (YPT1 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    S17C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3_HMOX1-V5-APEX2

    Plasmid

    #85823

    Purpose

    Expressed C-term tagged APEX2 on HMOX1 in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    HMOX1-V5-APEX2 (HMOX1 Human, Synthetic)
    

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Promoter

    CMV-F

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Per2:iLuc

    Plasmid

    #160293

    Purpose

    target Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.

    Depositor

    Joseph Takahashi

    Article

    Shan et al Neuron. 2020 Aug 4. pii: S0896-6273(20)3

    Insert

    Per2LUC
    

    Expression

    Mammalian

    Promoter

    no promotor

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CS2-Myc-hASUN

    Plasmid

    #47955

    Purpose

    Expression of tagged-human ASUN

    Depositor

    Laura Lee

    Article

    Jodoin et al Mol Biol Cell. 2013 Jul 31.

    Insert

    Asunder (INTS13 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    Sp6

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pCMV7.1_3xFLAG-STK4

    Plasmid

    #172989

    Purpose

    constitutive expression of FLAG-tagged STK4

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-STK4 (STK4 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 3xFlag-HaloTag-SUZ12 HDR - pDY082

    Plasmid

    #171110

    Purpose

    Homology directed repair plasmid for generating HaloTag-SUZ12 cells

    Depositor

    Thomas Cech

    Article

    Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

    Insert

    SUZ12 (SUZ12 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

    Plasmid

    #182825

    Purpose

    Cre-dependent expression of H2B-APEX fusion

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    H2B.APEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Promoter

    EF1a

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. eGFP-pk-S65A

    Plasmid

    #115619

    Purpose

    Expresses human Parkin S65A in mammalian cells

    Depositor

    Edward Fon

    Article

    Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

    Insert

    Parkin (PRKN Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S65A

    Promoter

    CMV

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. KIF5C(1-560)-2xmCherry-EF(C)

    Plasmid

    #61664

    Purpose

    Tandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)
    

    Expression

    Mammalian

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAT9752-BEAR-mScarlet

    Plasmid

    #162991

    Purpose

    BEAR target plasmid with split mScarlet and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mScarlet split with an intron between amino acids 110-111
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-SNAP-CRY2-VHH(GFP)

    Plasmid

    #58370

    Purpose

    For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (CRY2 Mustard Weed)
    

    Tags

    SNAP and vhhGFP4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pACDura3

    Plasmid

    #138711

    Purpose

    Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.

    Depositor

    Víctor de Lorenzo

    Article

    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

    Insert

    ura3 gene (URA3 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    optimized ura3 gene for expression in Escherichia…

    Promoter

    pEM7 promoter

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge