We narrowed to 7,375 results for: ef1a

1. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

   Plasmid

   #185060

   Purpose

   Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445

   Depositor

   Bobby Koeleman

   Insert

   U6:pegRNA:scaffold:PBS+RT template
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-DIO-mCherry-WPRE

   Plasmid

   #202620

   Purpose

   Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   mCherry
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lv EF1a HP1cs-Frb2x PGK Puro

   Plasmid

   #102809

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb2x fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   HP1cs-Frb2x
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA

   Plasmid

   #37086

   Purpose

   Expresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitation

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   ChETA-YFP
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC1553 - pAAV EF1a EGFP-KASH

   Plasmid

   #131682

   Purpose

   An adeno-associated viral vector expressing nuclear envelope-embedded eGFP

   Depositor

   Christopher Richie

   Article

   Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

   Insert

   EGFP-KASH
   

   Use

   AAV

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-EF1a-GFP-miRE_shRen-Blast

   Plasmid

   #135682

   Purpose

   Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

   Depositor

   Florian Karreth

   Insert

   shRenilla
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-CAG-NCreIntN-EF1a-mTagBFP2

   Plasmid

   #160507

   Purpose

   Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   NCreIntN
   

   Use

   Cre/Lox and Lentiviral

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-MinD-IRES-Puro

   Plasmid

   #213114

   Purpose

   Expression of untagged MinD in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-Coff/Fon-sRGECO

   Plasmid

   #137128

   Purpose

   Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-sRGECO
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   E217D

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-DIO-GRAB_g5-HT2m

    Plasmid

    #208722

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT2m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHage2-EF1a-mCherryTRF1-IRES-Blast

    Plasmid

    #182588

    Purpose

    expresses mcherry-trf1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTYF-EF1a-DLDH-IRES-EGFP

    Plasmid

    #178310

    Purpose

    Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in mammalian cells

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

    Insert

    D-lactate dehydrogenase
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC

    Plasmid

    #69587

    Purpose

    Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents dimerization and…

    Promoter

    EF1alpha

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-EF1a-AcGFP-P2A-hBRD4S

    Plasmid

    #197882

    Purpose

    study roles of human BET proteins in human pluripotency reprogramming

    Depositor

    Kejin Hu

    Article

    Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1A-LivePAR(R163A)-Hygro

    Plasmid

    #187609

    Purpose

    EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

    Depositor

    Robert Sobol

    Article

    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

    Insert

    LivePAR(R163A)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R163A mutation in the WWE Domain

    Promoter

    EF1A

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSicoR-Ef1a-mCh-Puro-GFPi

    Plasmid

    #31848

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-CCre-MBP4-WPRE

    Plasmid

    #193919

    Purpose

    Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    CCre-MBP4
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-NCre-MBP5-WPRE

    Plasmid

    #193918

    Purpose

    Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    NCre-MBP5
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFIN-EF1a-GFP:2A:CHER(HA)-WPRE

    Plasmid

    #44597

    Depositor

    Susan Semple-Rowland

    Article

    Verrier et al PLoS One. 2011;6(5):e20553. doi: 10.1371

    Insert

    EF1a-GFP:2A:CHER(HA)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human elongation factor -1a (pos 2528-3971)

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. H446 EF1a RA-ABE6.3 p2A Puro

    Plasmid

    #139104

    Purpose

    Lentiviral expression of ABE6.3.

    Depositor

    Wen Xue

    Article

    Song et al Nat Biomed Eng. 2019 Feb 25. pii: 10.103

    Insert

    ABE RA6.3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLenti-EF1a-MTS-FLAG-LOV*

    Plasmid

    #202208

    Purpose

    Plasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
    

    Use

    Lentiviral

    Tags

    FLAG and LOV*

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid

    #192585

    Purpose

    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    Lentiviral

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pYJ21-lenti-EF1a-ASAP2f-puro

    Plasmid

    #131065

    Purpose

    Forced expression of ASAP2f

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Stem Cell Res Ther. 2021 May 29;12(1):30

    Insert

    ASAP2f
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV-EF1a-FRT-EBFP-tTAn

    Plasmid

    #172127

    Purpose

    Expression of EBFP and tTA-InteinN in Flp recombinase positive cells

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    EBFP, tTAn-InteinN
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDH-CAG-IntCCCre-EF1a-Cerulean

    Plasmid

    #160508

    Purpose

    Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLV-REPORT(EF1a no GFP) mnuctdTomato

    Plasmid

    #172342

    Purpose

    mnuctdTomato

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSicoR-Ef1a-mCh-Puro-Luci

    Plasmid

    #31850

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    Luciferase RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN

    Plasmid

    #69582

    Purpose

    Expresses p53 tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    7 silent mutations in p53 DNA to prevent targetin…

    Promoter

    EF1alpha

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEF1a-RoninDHSA-FLAG-IRES-Neo

    Plasmid

    #28021

    Depositor

    Thomas Zwaka

    Article

    Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

    Insert

    Ronin (THAP11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    DHSA mutation (Y246A) of the previously defined H…

    Promoter

    Available Since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCDH-EF1a-tetR-T2A-tSapphire

    Plasmid

    #176312

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    T-sapphire
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge