Addgene

We narrowed to 9,524 results for: SUB

Showing: 1801 - 1850 of 9524 results

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Green Glifon50

Plasmid

#126206

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon50

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SC35-cMyc

Plasmid

#44721

Depositor

Kathleen Scotto

Article

Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

Insert

SC35 (SRSF2 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin4A (CUL4A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-RanQ69L

Plasmid

#30309

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

RanQ69L (RAN N/A)

Use

Tags

FLAG-Cherry

Expression

Mammalian

Mutation

Glutamine 69 is changed to Leucine

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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Lys-eMagA-EGFP

Plasmid

#162246

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

p18-eMagA-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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eMagA-EGFP-Mito

Plasmid

#162244

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagA-EGFP-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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ILID-CAAX

Plasmid

#85680

Purpose

Expression of untagged ILID CAAX

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Insert

ILID-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
eMagB-TgRFP-T

Plasmid

#162252

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagB-TgRFP-T

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-HA-hTAF4

Plasmid

#17983

Depositor

Naoko Tanese

Article

Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

Insert

TAF4 (TAF4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

missing 5' end

Promoter

Available since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
IL6-GFP

Plasmid

#28088

Depositor

Jennifer Stow

Article

Manderson et al J Cell Biol. 2007 Jul 2. 178(1):57-69.

Insert

IL6 (Il6 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2011

Availability

Academic Institutions and Nonprofits only

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PM-APEX2

Plasmid

#215557

Purpose

Stable, inducible expression in plasma membrane

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

Lyn11-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
eMagBF-TgRFPT

Plasmid

#162253

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagBF-TgRFPT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Syn-DIO-NaChBac-dTomato

Plasmid

#236252

Purpose

Cre dependent NaChBac-TdTomato expression

Depositor

Janelia Viral Tools

Article

Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

Insert

NaChBac-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xFLAG-V5-ccdB

Plasmid

#87064

Purpose

Mammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-PRAS40

Plasmid

#15481

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 (AKT1S1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

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ASAP2f

Plasmid

#83960

Purpose

Fluorescent reporter for voltage imaging

Depositor

Michael Lin

Article

Yang et al Cell. 2016 Jun 30;166(1):245-57. doi: 10

Insert

ASAP2f

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-P4Mx1

Plasmid

#108121

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCW01

Plasmid

#72300

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
negMaLionR

Plasmid

#113909

Purpose

negative control for Red ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCW02

Plasmid

#72301

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-HisD-miniGFP2

Plasmid

#188897

Purpose

Express 6xHis-Tag miniGFP2 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHT-ads

Plasmid

#47382

Purpose

Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Insert

ads

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Pgrac

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
eMagAF-EGFP-Mito

Plasmid

#162243

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagAF-EGFP-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 Flag-RagC

Plasmid

#99723

Purpose

Mammalian expression of Flag-RagC

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Use

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 PM-Ras-LOCKR-S

Plasmid

#216498

Purpose

FRET-based reporter for Ras activity, plasma membrane localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

PM-Ras-LOCKR-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-DN-hCUL5-FLAG

Plasmid

#15823

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin5 (CUL5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N441

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1 Flag BRG1

Plasmid

#1957

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BRG1 (SMARCA4 Human)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-DN-hCUL2-FLAG

Plasmid

#15819

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin2 (CUL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N427

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
negMaLionG

Plasmid

#113907

Purpose

negative control for Green ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPfetFn-Target_v2

Plasmid

#199564

Purpose

gRNA expression for FnCas12a in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

SacB

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pthl_fU

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LAMP1-APEX2

Plasmid

#215555

Purpose

Stable, inducible expression in lysosome

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

LAMP1-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
c-Rel cFlag pcDNA3

Plasmid

#20013

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Insert

c-Rel (Rel Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
Cyto-APEX2

Plasmid

#215552

Purpose

Stable, inducible expression in cytoplasm

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Ras-LOCKR-S

Plasmid

#216496

Purpose

FRET-based reporter for Ras activity, untargeted

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Ras-LOCKR-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 Flag-RagC(S75N)

Plasmid

#99724

Purpose

Mammalian expression of Flag-RagC(S75N)

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Use

Tags

Flag

Expression

Mutation

S75N

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5_FRT_mCH

Plasmid

#127109

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 Flag-RagA

Plasmid

#99714

Purpose

Mammalian expression of Flag-RagA

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Use

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CA

Plasmid

#69616

Purpose

To express gene strongly with AAV

Depositor

Naoshige Uchida

Article

Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

Type

Empty backbone

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/HisB-PAmKate

Plasmid

#32691

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Use

Tags

Expression

Bacterial

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

araB

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-HA-hTAF6

Plasmid

#17988

Depositor

Naoko Tanese

Article

Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

Insert

TAF6 (TAF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
MaLionB

Plasmid

#113910

Purpose

Blue ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3_UnaG-Flag-Sec61B

Plasmid

#83413

Purpose

Expressed N-term tagged UnaG on Sec61B

Depositor

Hyun-Woo Rhee

Article

Park et al ACS Chem Biol. 2016 May 27.

Insert

UnaG-Sec61B (SEC61B Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-mBAF45a IRES GFP

Plasmid

#17880

Depositor

Jerry Crabtree

Article

Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

Insert

BAF45a (Phf10 Mouse)

Use

Tags

FLAG and IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pACAGW-ChR2-Venus-AAV

Plasmid

#20071

Purpose

AAV-mediated expression of ChR2-Venus for neuronal excitation

Depositor

Karel Svoboda

Article

Leopoldo et al Nature. 2009 Jan 18.

Has Service

AAV1 and AAV9

Insert

Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)

Use

AAV

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CAGG

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW yAT1.03

Plasmid

#132781

Purpose

FRET biosensor for ATP measurements in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

Insert

yAT1.03 ymTq2-tdTomato

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Golgi-Ras-LOCKR-S

Plasmid

#216499

Purpose

FRET-based reporter for Ras activity, Golgi localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Golgi-Ras-LOCKR-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 ER-Ras-LOCKR-S

Plasmid

#216500

Purpose

FRET-based reporter for Ras activity, ER localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

ER-Ras-LOCKR-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Mito-Ras-LOCKR-S

Plasmid

#216501

Purpose

FRET-based reporter for Ras activity, mitochondria localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Mito-Ras-LOCKR-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NES-mTagBFP2-P4Mx1

Plasmid

#108142

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):mTagBFP2:L. penomophila SidM(546-647)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1801 - 1850 of 9524 results

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