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pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

Plasmid

#176458

Purpose

Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

Depositor

Hui Yang

Article

Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

Insert

humanized S. pyogenes Cas9-XTEN-Ec73RT fusion

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
pLKO.1-sh-mSlug-3

Plasmid

#40647

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSlug-3 (Snai2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pAB07 (CK2beta)

Plasmid

#27091

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Tags

Myc

Expression

Mammalian

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only
pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry

Plasmid

#139649

Purpose

Reporter assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

H2B-mCherry

Use

Lentiviral

Tags

FLAG, His6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-GFI1B

Plasmid

#16169

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Growth factor independent 1B (GFI1B Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
attB53-Pac-TRE-3xFlag-Neurog1-attB53

Plasmid

#183613

Purpose

RMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

Neurog1 (Neurog1 Mouse)

Use

Rmce

Tags

3xFlag

Expression

Mammalian

Promoter

TRE

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_EIF2AK2

Plasmid

#106108

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting EIF2AK2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting EIF2AK2 (EIF2AK2 Human)

Use

CRISPR

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS

Plasmid

#187358

Purpose

Lentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tag

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

HPC-4 antibody heavy chain, and 6xHIS tag

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-2xmCitrine-GFP11

Plasmid

#61667

Purpose

Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCRE-iRFP670

Plasmid

#82696

Purpose

Expresses CRE and iRFP670 under two separate PGK promoters.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Insert

CRE, iRFP670

Use

Cre/Lox

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNATM3.1/V5-His TOPO-PKCi-K274W

Plasmid

#55690

Purpose

expresses dominant negative human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Tags

V5/His

Expression

Mammalian

Mutation

A120E and K274W

Promoter

CMV

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only
yPet_cp229-Binder

Plasmid

#179137

Purpose

YPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

SspB

Tags

yPet_cp229

Expression

Mammalian

Mutation

Y11K/A15E, residue numbering from pdb: 1ou9

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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FOR human NANOS3 TALEN

Plasmid

#59722

Purpose

TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG

Use

TALEN

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
REV human NANOS3 TALEN

Plasmid

#59723

Purpose

TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI

Use

TALEN

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1 shJUND puro

Plasmid

#58698

Purpose

Lentiviral shRNA vector for knockdown of human JUND

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

shJUND (JUND Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
pLentiPGK Puro DEST JNKKTR EE Clover

Plasmid

#90239

Purpose

Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (EE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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mEmerald-MyosinIIA-C-18

Plasmid

#54190

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

Insert

MyosinIIA

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
iRFP713-N2-Frankenbody-Flag

Plasmid

#175291

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xFLAG tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with iRFP713

Tags

iRFP713

Expression

Mammalian

Available Since

Dec. 3, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-mCherry-attP(C31)-*BleoR

Plasmid

#183752

Purpose

phiC31 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

FRT-mCherry-attP-BleoR-F3

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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