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lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry
Plasmid#202822PurposeLentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expressionDepositorInserttwo sgRNAs that delete exon 1 within the ZNF91 gene
UseLentiviralTagsExpressionMammalianMutationPromoterU6 - twoAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pLINK cyclin T1 (1-280) myc (K93D) (P#580)
Plasmid#14611DepositorInsertcyclin T1 (1-280) K93D (CCNT1 Human)
UseTagsmycExpressionMammalianMutationK93D. Deficient in binding to CDK9. Inactive in 3…PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEF6-Lck-TurboID
Plasmid#159433Purposeexpresses Lck-TurboID in mammalian cellsDepositorInsertsUseTagsHAExpressionMammalianMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterEF-1aAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGPN-ACE2
Plasmid#187483PurposeExpression vector - ACE2DepositorInsertACE2 (ACE2 Human)
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221E
Plasmid#108108PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-195-3p
Plasmid#103311PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-195-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-195-3p target (MIR195 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29a-3p
Plasmid#103391PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29a-3p target (MIR29A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-PGD2-1.1
Plasmid#214764PurposeAAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoterDepositorInsertGRAB-PGD2-1.1 sensor
UseAAVTagsExpressionMutationPromoterEF-1-alpha promoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_Sik3_T142Q
Plasmid#119973PurposeLentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance geneDepositorInsertSik3 (Sik3 Mouse)
UseLentiviralTagsExpressionMammalianMutationchange Threonine 142 to GlutaminePromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pPKm-248
Plasmid#90504PurposeExpresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tPCYA - IRES - MTS - tHO1 - P2A - MTS - tFD - P2A - MTS - tFNRDepositorInsertmito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EEF1A2_GTP-EFTU
Plasmid#109862PurposeProtein expression and purification of EEF1A2_GTP-EFTUDepositorInsertEEF1A2_GTP-EFTU (EEF1A2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29a-5p
Plasmid#103392PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29a-5p target (MIR29A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-IFITM3g1 (BB15)
Plasmid#139460PurposeLentiviral vector with gRNA targeting IFITM3; includes puromycin selectable markerDepositorInsertIFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
SaLgCP
Plasmid#164562PurposePuroDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsFlag-NLS-SaCas9-P2A-PuroRExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
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LentiGuide-Puro-NCOA7g1 (BB22)
Plasmid#139457PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g2 (BB23)
Plasmid#139458PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-mCherryTRF1-IRES-Blast
Plasmid#182588Purposeexpresses mcherry-trf1 in mammalian cellsDepositorInserthTRF1 (TERF1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
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Cre-responsive Cas9 expressing transposon library vector
Plasmid#164138PurposeBackbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6, EF-1aAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-331-3p
Plasmid#103444PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-331-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-331-3p target (MIR331 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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LentiV_Neo_SIK3_CR_K95M
Plasmid#108106PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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LSB-hsa-miR-296-3p
Plasmid#103387PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-296-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-296-3p target (MIR296 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
hEBI3 pEF6-V5 (MJS_002)
Plasmid#72490PurposeExpression of human EBI3 in mammalian cells (V5 tag)DepositorInsertEBI3 (EBI3 Human)
UseTagsHis/V5ExpressionMammalianMutationPromoterEF-1 alphaAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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LSB-hsa-miR-485-3p
Plasmid#103553PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-485-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-485-3p target (MIR485 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7a-3p
Plasmid#103145PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7a-3p target (MIRLET7A3 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-146a-3p
Plasmid#103247PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-146a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-146a-3p target (MIR146A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-221-5p
Plasmid#103366PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-5p target (MIR221 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-221-3p
Plasmid#103365PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-3p target (MIR221 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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LSB-hsa-miR-150-5p
Plasmid#103257PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-150-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-150-5p target (MIR150 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-223-3p
Plasmid#103369PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-223-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-223-3p target (MIR223 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only
