167,820 results

1. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

   Plasmid

   #139093

   Purpose

   Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   dxCas9-NG-VPR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. WDR59 in pRK5

   Plasmid

   #154214

   Purpose

   Untagged WDR59 cDNA expression vector for transfections

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   WDR59 (WDR59 Human)
   

   Tags

   NONE

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCVpuro-Bcl2

   Plasmid

   #191964

   Purpose

   Exogenous expression of Bcl2 WT

   Depositor

   Luca Scorrano

   Insert

   Bcl2 (BCL2 Human)
   

   Use

   Retroviral

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCMV-intron myc Rab2WT

   Plasmid

   #46779

   Purpose

   expression of WT Rab2a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB2 (RAB2A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pSico

   Plasmid

   #11578

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEmerald-Clathrin-15

   Plasmid

   #54040

   Purpose

   Localization: Clathrin Vesicles, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

   Insert

   Clathrin (CLTB Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

   Plasmid

   #209078

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   3K mutant: E35K, E46K, E61K

   Promoter

   TRE4G

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLG005 (Retron-TIR)

   Plasmid

   #157883

   Purpose

   Expresses the retron-TIR defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   Retron-TIR
   

   Expression

   Bacterial

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC18T-mini-Tn7T-Tp-ecfp

   Plasmid

   #65034

   Purpose

   TpR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   ecfp
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGT-S1

    Plasmid

    #122582

    Purpose

    MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    beta-lactamase/sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS_nanorod_105b

    Plasmid

    #120347

    Purpose

    phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

    Insert

    Staple sequences for a DNA origami nanorod
    

    Expression

    Bacterial

    Promoter

    none

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLQ1651-sgRNA(F+E)-sgGal4

    Plasmid

    #100549

    Purpose

    sgGal4-expressing vector modified from Addgene plasmid #51024

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    sgGal4
    

    Use

    CRISPR

    Tags

    sgRNA

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET24b-APEX2

    Plasmid

    #111702

    Purpose

    For IPTG-inducible expression of APEX2 in E. coli

    Depositor

    Jessica Seeliger

    Article

    Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

    Insert

    APEX2
    

    Tags

    6xHis and V5

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-EZH2

    Plasmid

    #116738

    Purpose

    Lentiviral expression of EZH2

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSGNluc

    Plasmid

    #186705

    Purpose

    A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

    Depositor

    Maria Leptin, Baubak Bajoghli

    Article

    Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

    Inserts

    GFP
    luciferase
    

    Available Since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6/V5-DEST-RNF2

    Plasmid

    #31216

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    RNF2 (RNF2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

17. GFP-NPM WT

    Plasmid

    #17578

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_V5-NEUROD1

    Plasmid

    #215031

    Purpose

    Mammalian expression of human NEUROD1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    neuronal differentiation 1 (NEUROD1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDESTpK7WG2mito

    Plasmid

    #158726

    Purpose

    Plant mitoTALEN vector assembly, Destination vector

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    mitochondrial presequence in front of attR1 in pk7WG2
    

    Expression

    Plant

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. GreenGate 2.0 Toolkit

    Plasmid Kit

    #1000000241

    Purpose

    The GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.

    Depositor

    Alexis Maizel

    Available Since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only