213,586 results

1. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

   Plasmid

   #138152

   Purpose

   Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   eGFP-MCS
   

   Use

   Lentiviral

   Tags

   2PEST AND NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKrox24(2xD-E)dTomato

   Plasmid

   #200110

   Purpose

   Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element, no other promoter elemen…

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p006-Banana-Late

   Plasmid

   #112251

   Purpose

   ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   ATF1 under osmY promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   osmY

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

   Plasmid

   #100848

   Purpose

   AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1 and AAV9

   Insert

   jRCaMP1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GST-Cdk5

   Plasmid

   #24894

   Depositor

   Harish Pant

   Article

   Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

   Insert

   CDK5 (CDK5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 17, 2010

   Availability

   Academic Institutions and Nonprofits only

6. GST-Cdk5

   Plasmid

   #24894

   Depositor

   Harish Pant

   Article

   Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

   Insert

   CDK5 (CDK5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 17, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pCMMP-MCS-IRES-eGFP-P2A-iCas9

   Plasmid

   #178533

   Purpose

   For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

   Depositor

   Ryoji Yao

   Article

   Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

   Insert

   iCaspase9 (CASP9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3336 pcDNA3 Bcl-2

   Plasmid

   #8768

   Depositor

   Stanley Korsmeyer

   Article

   Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 3336 pcDNA3 Bcl-2

   Plasmid

   #8768

   Depositor

   Stanley Korsmeyer

   Article

   Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pME-GAL4-ERT2-VP16

    Plasmid

    #82588

    Purpose

    Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

    Depositor

    Kryn Stankunas

    Article

    Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

    Insert

    GAL4-ERT2-VP16
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDB_345

    Plasmid

    #231113

    Purpose

    ABE activity-based selection cloning vector

    Depositor

    David Root, John Doench

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. TurboID-EV

    Plasmid

    #215085

    Purpose

    Expresses TurboID in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    TurboID (birA )
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV pCAG-FLEX-EGFP-WPRE

    Plasmid

    #51502

    Purpose

    Can be used to generate AAV virus that will express EGFP in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV pCAG-FLEX-EGFP-WPRE

    Plasmid

    #51502

    Purpose

    Can be used to generate AAV virus that will express EGFP in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HTR2A-Tango

    Plasmid

    #66409

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR2A (HTR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HTR2A-Tango

    Plasmid

    #66409

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR2A (HTR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PL-SIN-EOS-C(3+)-EiP

    Plasmid

    #21313

    Purpose

    Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EOS-C(3+) promoter region

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PL-SIN-EOS-C(3+)-EiP

    Plasmid

    #21313

    Purpose

    Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EOS-C(3+) promoter region

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223-CSNK2A1

    Plasmid

    #23678

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pDONR223-CSNK2A1

    Plasmid

    #23678

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pPAmCherry-a-tubulin

    Plasmid

    #31930

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    a-tubulin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPAmCherry-a-tubulin

    Plasmid

    #31930

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    a-tubulin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBabe_puro_DEST_Flag_PLD1

    Plasmid

    #45268

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    PLD1 (PLD1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBabe_puro_DEST_Flag_PLD1

    Plasmid

    #45268

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    PLD1 (PLD1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUCmini-iCAP-PHP.AX

    Plasmid

    #195218

    Purpose

    AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)

    Depositor

    Viviana Gradinaru

    Article

    Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103

    Insert

    Synthetic construct isolate AAV-PHP.AX VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-G-Creon shRNA[Control]

    Plasmid

    #181824

    Purpose

    This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

    Depositor

    Eun Mi Hwang

    Article

    Kim et al J Neurosci Methods. 2021 Dec 21:109452.

    Type

    Empty backbone

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. V48 ELP

    Plasmid

    #68395

    Purpose

    Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    V48 ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. V48 ELP

    Plasmid

    #68395

    Purpose

    Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    V48 ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-SUN2 mScarlet

    Plasmid

    #187685

    Purpose

    In vivo visualization of SUN2

    Depositor

    Mary Beckerle

    Insert

    SUN2 (Sun2 Mouse)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Halo-KDEL

    Plasmid

    #124316

    Purpose

    Expresses ER specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Use

    Tags

    KDEL Seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge