218,492 results

1. psd44-GqQL

   Plasmid

   #46826

   Purpose

   2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   g protein alpha q (GNAQ Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Q209L (The mutation reduces GTPase activity resul…

   Promoter

   PGK promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-2T PTP-1B

   Plasmid

   #8602

   Depositor

   Ben Neel

   Insert

   PTP-1B (PTPN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWUR790

   Plasmid

   #101723

   Purpose

   Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

   Depositor

   John van der Oost

   Article

   Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

   Insert

   PfAgo
   

   Use

   Tags

   Strep(II)-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promotor

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSH62-EBD

   Plasmid

   #49455

   Purpose

   yeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinase

   Depositor

   Marc Gartenberg

   Article

   Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

   Insert

   Cre-EBD fusion
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1 promoter

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCX-ThPDE5VV

   Plasmid

   #134778

   Purpose

   cGMP FRET biosensor

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

   Insert

   ThPDE5VV
   

   Use

   Tags

   cpVenus-Venus and mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQC membrane TdTomato IX

   Plasmid

   #37351

   Depositor

   Connie Cepko

   Article

   Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

   Insert

   membrane TdTomato
   

   Use

   Tags

   Palmitoylation signal*

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL-MS2-6X

   Plasmid

   #27118

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2 binding sites (6)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQCXIP-GFP1-10

   Plasmid

   #68715

   Purpose

   Split GFP assay

   Depositor

   Yutaka Hata

   Article

   Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

   Insert

   GFP1-10
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-myc-Atg7

   Plasmid

   #24921

   Depositor

   Toren Finkel

   Article

   Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

   Insert

   Atg7 (ATG7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMVTNT-T7-KPNA2

    Plasmid

    #26678

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 . 11():63.

    Insert

    Karyopherin Alpha 2 (KPNA2 Human)
    

    Use

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. Mouse SLC KO Library

    Pooled Library

    #141436

    Purpose

    Expresses gRNAs for knockout of the mouse Solute Carrier family

    Depositor

    Giulio Superti-Furga

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR and Lentiviral

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

12. paavCAG-post-mGRASP-2A-dTomato

    Plasmid

    #34912

    Purpose

    post

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Insert

    postsynaptic mGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV HA hRB-wt

    Plasmid

    #58905

    Purpose

    mammalian expression of wild type human Rb

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.0 FLAG TRPV4

    Plasmid

    #45751

    Depositor

    Robert Lefkowitz

    Article

    Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

    Insert

    TRPV4 (Trpv4 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBB550

    Plasmid

    #27396

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 . 7():32.

    Insert

    dnaK, dnaJ, GroESL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2 mCherry-Rtn4a

    Plasmid

    #86683

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4a (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-mEOS3.2-KanMX6

    Plasmid

    #87030

    Purpose

    Tag S. pombe genes in C terminal with mEOS3.2

    Depositor

    Thomas Pollard, Julien Berro

    Article

    Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    mEOS3.2
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti-EF1a-dCas9-VP64-Puro

    Plasmid

    #99371

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-VP64-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUCP20T-tdTomato

    Plasmid

    #78469

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHAGE-MET

    Plasmid

    #116762

    Purpose

    Lentiviral expression of MET

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MET (MET Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TFORF0070

    Plasmid

    #141505

    Purpose

    Lentiviral vector for overexpressing the CSDE1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CSDE1 (CSDE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5-HA-UFM1

    Plasmid

    #134639

    Purpose

    expression of HA-tagged human UFM1 wildtype

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFM1 (UFM1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGP-CMV-NES-jRCaMP1b

    Plasmid

    #63136

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRCaMP1b
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. tdTomato-Keratin-17

    Plasmid

    #58106

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green

    Plasmid

    #170048

    Purpose

    Tri-Responsive Reporter Gene Construct for redgreen, triple red, and double green

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet
    COmScarlet
    mRuby3
    mWasabi dN
    mWasabi CO
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDG1-PHP.eB

    Plasmid

    #220799

    Purpose

    AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    E4, E2A, VA, AAV2 rep and PHP.eB cap genes
    

    Use

    AAV; Aav helper vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pQF2WB

    Plasmid

    #61312

    Purpose

    Enhancer trap version of QF2, analogous to pGAWB

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mPlum-EB1-8

    Plasmid

    #55963

    Purpose

    Localization: MT End Binding Protein, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

30. PJEBAN6

    Plasmid

    #203684

    Purpose

    Expresses red fluorescent marker DsRed-Express in Listeria monocytogenes

    Depositor

    Tine Rask Licht

    Article

    Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

    Insert

    DsRed-Express
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge