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Showing: 121 - 160 of 21684 results

  1. Desmoplakin I tension sensor (F40-based)

    Plasmid
    #118725
    Purpose
    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[YPet(short)-F40-mCherry] (internal-1952) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118725-plasmid-map-sequence-id-230441

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  2. Actin Binding Domain Tension Sensor

    Plasmid
    #215368
    Purpose
    Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081
    Insert
    Actin Binding Domain Tension Sensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    215368-plasmid-map-sequence-id-430713

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  3. Actin Binding Domain Tensionless Control

    Plasmid
    #215371
    Purpose
    Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081
    Insert
    Actin Binding Domain Tensionless Control
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    215371-plasmid-map-sequence-id-430717

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  5. Desmoplakin I tension sensor (F40-based)

    Plasmid
    #119186
    Purpose
    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119186-plasmid-map-sequence-id-230533

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  8. Desmoplakin II tension sensor (F40-based)

    Plasmid
    #118715
    Purpose
    The F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-F40-mCherry] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118715-plasmid-map-sequence-id-230444

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  10. pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2

    Plasmid
    #186361
    Purpose
    Entry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Human Ensconsin (MAP7 Synthetic, Human)
    Use
    Expression of a fluorescent microtubule marker
    Tags
    3xEGFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186361-plasmid-map-sequence-id-373431

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  11. Desmoplakin I tension sensor (F40-based)

    Plasmid
    #118725
    Purpose
    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[YPet(short)-F40-mCherry] (internal-1952) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118725-plasmid-map-sequence-id-230441

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  13. pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)

    Plasmid
    #49060
    Purpose
    Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP (chloride-sensitive)
    Expression
    Mammalian
    Mutation
    Cl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…
    Promoter
    CMV
    Available since
    Nov. 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    49060-plasmid-map-sequence-id-72178

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  23. pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)

    Plasmid
    #49060
    Purpose
    Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP (chloride-sensitive)
    Expression
    Mammalian
    Mutation
    Cl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…
    Promoter
    CMV
    Available since
    Nov. 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    49060-plasmid-map-sequence-id-72178

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  34. Desmoplakin I affinity mutant (F40-based tension sensor)

    Plasmid
    #119190
    Purpose
    The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119190-plasmid-map-sequence-id-232293

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  35. Cre-responsive Cas9 expressing transposon library vector

    Plasmid
    #164138
    Purpose
    Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)
    Depositor
    Hyongbum Kim
    Article
    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00
    Type
    Empty backbone
    Use
    CRISPR, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6, EF-1a
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    164138-plasmid-map-sequence-id-333975

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  37. Desmoplakin II donor only control (FL-based tension sensor)

    Plasmid
    #118723
    Purpose
    The donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    inserted FL-based tension sensor module after aa1…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118723-plasmid-map-sequence-id-230442

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Showing: 121 - 160 of 21684 results