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We narrowed to 16,877 results for: Cas9

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Showing: 61 - 80 of 16877 results

  4. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid
    #42337
    Purpose
    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42337-plasmid-map-sequence-id-57166

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  8. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

    Plasmid
    #64222
    Purpose
    Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A
    Depositor
    Ralf Kuehn
    Publication
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    sgRNA cassette
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    May 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64222-plasmid-map-sequence-id-107958

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  12. lentiCas9-Blast

    Plasmid
    #52962
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has service
    Lentiviral Prep
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52962-plasmid-map-sequence-id-322376

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  13. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65767-plasmid-map-sequence-id-471631

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  14. pSECC

    Plasmid
    #60820
    Purpose
    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre
    Depositor
    Tyler Jacks
    Publication
    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature
    Inserts
    Cas9
    Cre
    Use
    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    BsmBI site eliminated by C->A; E308G
    Promoter
    EFS and EFS (after Cas9-2A)
    Available since
    Nov. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60820-plasmid-map-sequence-id-371636

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  16. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Publication
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67639-plasmid-map-sequence-id-220324

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  18. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    43861-plasmid-map-sequence-id-59200

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  19. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Publication
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90085-plasmid-map-sequence-id-182691

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Showing: 61 - 80 of 16877 results