We narrowed to 14,481 results for: cas9

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p201H Cas9

Plasmid

#59176

Purpose

Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
hph

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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p201N Cas9

Plasmid

#59175

Purpose

Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
nptII

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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Cas9-NAT

Plasmid

#64329

Purpose

Cas9 expression cassette carried by a yeast single-copy episome plasmid

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette

Use

CRISPR

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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p201B Cas9

Plasmid

#59177

Purpose

Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
BAR

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9 NGFR

Plasmid

#155279

Purpose

Lentiviral Cas9 expression with NGFR marker

Depositor

Martin Carroll

Insert

spCas9

Expression

Mammalian

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pHsp70-Cas9

Plasmid

#45945

Purpose

The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2013 May 24.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

3x FLAG

Mutation

Silent change from C to A at bp 834 of insert

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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p201G Cas9

Plasmid

#59178

Purpose

Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
sGFP

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9

Plasmid

#128160

Purpose

Ptet-controlled expression of S. pyogenes Cas9

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Promoter

Ptet

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Promoter

U6 and CBh

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mAMPKa1

Plasmid

#79004

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa1 (Prkaa1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pCS2-Cas9

Plasmid

#47322

Purpose

For in vivo expression of Cas9 or generation of Cas9 mRNA

Depositor

Alex Schier

Article

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR; Xenopus and zebrafish expression

Expression

Mammalian

Promoter

SP6

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mAMPKa2

Plasmid

#79005

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa2 (Prkaa2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pX2-Cas9

Plasmid

#85811

Purpose

Arabinose inducible Cas9 in a broad host range backbone.

Depositor

Ryan Gill

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-Cas9

Plasmid

#89995

Purpose

Expresses high levels of WT Cas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

Insert

hCas9

Tags

3xFlag, SV40 NLS, and T2A peptide

Expression

Mammalian

Promoter

CAG

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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Cas9 Null

Plasmid

#198727

Purpose

Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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AAEL005635-Cas9

Plasmid

#100608

Purpose

The promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL005635-hspcas9-GFP
Opie2-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL005635 and Opie2

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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PE-Cas9

Plasmid

#174851

Purpose

Express PE-Cas9 in mammalian cells

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

PE-Cas9

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Sniper-Cas9

Plasmid

#138559

Purpose

Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

Sniper-Cas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

F539S, M763I, K890N

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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modRNAc0-Cas9

Plasmid

#170180

Purpose

IVT template plasmid for making modified Cas9 RNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

Cas9

Use

Ivt template plasmid

Promoter

T7 promoter

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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